Anti-Von Hippel Lindau antibody - N-terminal

• Supplier: Abcam
• Catalog: ab135576
• Prices: $384.00
• Sizes: 400 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 50-79 of Human Von Hippel Lindau
• Description: Rabbit Polyclonal
• Gene: VHL
• Conjugate: Unconjugated

Product images

Anti-Von Hippel Lindau antibody - N-terminal (ab135576) at 1/50 dilution + HepG2 cell line lysates at 35 µgdeveloped using the ECL technique

Immunohistochemical analysis of formalin fixed, paraffin embedded Mouse brain tissue labelling Von Hippel Lindau with ab135576 at 1/50. Detection utilised peroxidase conjugation to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of HepG2 cells labelling Von Hippel Lindau with ab135576 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC conjugated Goat anti Rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of HepG2 cells labelling Von Hippel Lindau with ab135576 at 1/10 followed by Alexa Fluor 488 conjugated Goat anti Rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).