Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Von Willebrand Factor with ab9378.
Anti-Von Willebrand Factor antibody (ab9378) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Central Panel: ab9378 staining Von Willebrand Factor in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 568-conjugated Goat polyclonal to rabbit IgG, dilution 1/100, was used as secondary antibody. Top panel: Nuclei counterstained with DAPI (blue).Bottom panel: OverlaySee Abreview
ab9378 staining Von Willebrand Factor in Human platelet cells by Flow cytometry.Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution. P : Permeabilized; US : Unstained, Red Peak; IGG RB : IgG Rabbit (Isotype Control), Blue Peak; VWF : Von Willebrand Factor antibody, Green peak.See Abreview
Human normal colon. Staining is localised to storage granules. Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.