Anti-VPAC2 antibody

• Supplier: Abcam
• Catalog: ab28624
• Prices: $379.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide: LQFHRGSRAQSFLQTETSVI conjugated to KLH, corresponding to amino acids 419-438 of Human VIP Receptor 2 Run BLAST with Run BLAST with
• Description: Rabbit Polyclonal
• Gene: VIPR2
• Conjugate: Unconjugated

Product images

ab28624 staining Human VIP Receptor 2 in human neuroendocrine cells of the small intestine by immunohistochemistry using paraffin embedded tissue.

ICC/IF image of ab28624 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28624, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian - Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian

Miller JE, Granados-Fuentes D, Wang T, Marpegan L, Holy TE, Herzog ED. J Neurosci. 2014 Apr 23;34(17):6040-6.

VPAC receptor mediated tumor cell targeting by protamine based nanoparticles. - VPAC receptor mediated tumor cell targeting by protamine based nanoparticles.

Ortner A, Wernig K, Kaisler R, Edetsberger M, Hajos F, Kohler G, Mosgoeller W, Zimmer A. J Drug Target. 2010 Jul;18(6):457-67.