Anti-VPS24 antibody
| Name | Anti-VPS24 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab76333 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide from the N terminal region of human VPS24, conjugated to an immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | CHMP3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-VPS24 antibody (ab76333) at 1/1000 dilutionLane 1 : Rat Brain lysatesLane 2 : Rat Brain lysates + ab76333 pre-absorbed with immunizing peptidePerformed under reducing conditions.
ICC/IF image of ab76333 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76333, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.