Anti-VPS26 antibody
| Name | Anti-VPS26 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23892 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human VPS26 |
| Blocking Peptide | Human VPS26 peptide |
| Description | Rabbit Polyclonal |
| Gene | VPS26A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-VPS26 antibody (ab23892) at 1 µg/mlLane 1 : Human kidney lysateLane 2 : Human kidney lysate with Human VPS26 peptide (ab24288) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryAlexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilutionPerformed under reducing conditions.
Image courtesy of Human Protein Atlasab23892 staining VPS26 in Human kidney. The paraffin embedded tissue was incubated with ab23892 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23892 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell linesFurther results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab23892 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23892, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HepG2 cells.
All lanes : Anti-VPS26 antibody (ab23892) at 1/1000 dilutionLane 1 : Rat NRK whole cell lysate at 25 µgLane 2 : Rat NRK whole cell lysate at 50 µgSecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-VPS26 antibody (ab23892) at 1/1000 dilutionLane 1 : HEK293T whole cell lysateLane 2 : HeLa whole cell lysateLysates/proteins at 50 µg per lane.SecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunofluorescence analysis of HeLa cells treated with mDpy-30 siRNA, staining VPS26 (green) with ab23892. Cells were fixed formaldehyde, permeabilized and blocked with 5% goat serum for 20 minutes. Samples were incubated with primary antibody in blocking buffer for 2 hours before incubating with fluorophore-conjugated secondary antibody for 1 hour. Nuclei were detected using DAPI.
Product References
Mutation in VPS35 associated with Parkinson's disease impairs WASH complex - Mutation in VPS35 associated with Parkinson's disease impairs WASH complex
Zavodszky E, Seaman MN, Moreau K, Jimenez-Sanchez M, Breusegem SY, Harbour ME, Rubinsztein DC. Nat Commun. 2014 May 13;5:3828.
VPS35 dysfunction impairs lysosomal degradation of alpha-synuclein and - VPS35 dysfunction impairs lysosomal degradation of alpha-synuclein and
Miura E, Hasegawa T, Konno M, Suzuki M, Sugeno N, Fujikake N, Geisler S, Tabuchi M, Oshima R, Kikuchi A, Baba T, Wada K, Nagai Y, Takeda A, Aoki M. Neurobiol Dis. 2014 Nov;71:1-13.
Regulation of mammalian autophagy by class II and III PI 3-kinases through PI3P - Regulation of mammalian autophagy by class II and III PI 3-kinases through PI3P
Devereaux K, Dall'Armi C, Alcazar-Roman A, Ogasawara Y, Zhou X, Wang F, Yamamoto A, De Camilli P, Di Paolo G. PLoS One. 2013 Oct 3;8(10):e76405.
The role of ceroid lipofuscinosis neuronal protein 5 (CLN5) in endosomal sorting. - The role of ceroid lipofuscinosis neuronal protein 5 (CLN5) in endosomal sorting.
Mamo A, Jules F, Dumaresq-Doiron K, Costantino S, Lefrancois S. Mol Cell Biol. 2012 May;32(10):1855-66.
Modulation of cell adhesion and migration by the histone methyltransferase - Modulation of cell adhesion and migration by the histone methyltransferase
Xia B, Joubert A, Groves B, Vo K, Ashraf D, Djavaherian D, Awe J, Xiong Y, Cherfils J, Ma D. PLoS One. 2010 Jul 23;5(7):e11771.
Regulation of P2Y1 receptor traffic by sorting Nexin 1 is retromer independent. - Regulation of P2Y1 receptor traffic by sorting Nexin 1 is retromer independent.
Nisar S, Kelly E, Cullen PJ, Mundell SJ. Traffic. 2010 Apr;11(4):508-19.
Sorting nexin 8 regulates endosome-to-Golgi transport. - Sorting nexin 8 regulates endosome-to-Golgi transport.
Dyve AB, Bergan J, Utskarpen A, Sandvig K. Biochem Biophys Res Commun. 2009 Dec 4;390(1):109-14. doi:
Dynamic proteomics of individual cancer cells in response to a drug. - Dynamic proteomics of individual cancer cells in response to a drug.
Cohen AA, Geva-Zatorsky N, Eden E, Frenkel-Morgenstern M, Issaeva I, Sigal A, Milo R, Cohen-Saidon C, Liron Y, Kam Z, Cohen L, Danon T, Perzov N, Alon U. Science. 2008 Dec 5;322(5907):1511-6.