Anti-VPS28 antibody [EPR9880]

• Supplier: Abcam
• Catalog: ab154793
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9880
• Applications: WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human VPS28
• Description: Rabbit Monoclonal
• Gene: VPS28
• Conjugate: Unconjugated

Product images

Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified) + HepG2 cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified) + HepG2 cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution (unpurified)Lane 1 : HepG2 cell lysateLane 2 : Jurkat cell lysateLane 3 : LnCaP cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.