VPS28

Gene Symbol VPS28
Entrez Gene 51160
Alt Symbol -
Species Human
Gene Type protein-coding
Description vacuolar protein sorting 28 homolog (S. cerevisiae)
Other Description ESCRT-I complex subunit VPS28|vacuolar protein sorting-associated protein 28 homolog|yeast class E protein Vps28p homolog
Swissprots Q9UK41 Q86VK0
Accessions EAW82094 EAW82095 EAW82096 EAW82097 EAW82098 EAW82099 EAW82100 Q9UK41 AF182844 AAF00499 AF316887 AAK00314 AF445417 AAQ04641 AK026865 AK090942 AK091979 AK310062 BC006485 AAH06485 BC019321 AAH19321 BC050438 BC050712 BC050713 AAH50713 BP380505 BU658837 JF432621 ADZ15838 N31929 R72904 XM_005272323 XP_005272380 XM_005272324 XP_005272381 XM_005272325 XP_005272382 XM_011517099 XP_011515401 XM_011517100 XP_011515402 NM_016208 NP_057292 NM_183057 NP_898880
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process. {ECO:0000269|PubMed:11916981}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:11916981}. Late endosome membrane {ECO:0000269|PubMed:11916981}; Peripheral membrane protein {ECO:0000269|PubMed:11916981}.
Top Pathways Endocytosis

VPS28 (B-2) - sc-376337 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS28 (B-2): sc-376337. Western blot analysis of VPS28 expression in non-transfected: sc-117752 (A) and mouse VPS28 transfected: sc-124578 (B) 293T whole cell lysates.

VPS28 (E-10) - sc-166514 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS28 (E-10): sc-166514. Western blot analysis of VPS28 expression in non-transfected: sc-117752 (A) and mouse VPS28 transfected: sc-124578 (B) 293T whole cell lysates.

VPS28 (E-7) - sc-166537 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS28 (E-7): sc-166537. Western blot analysis of VPS28 expression in non-transfected: sc-117752 (A) and mouse VPS28 transfected: sc-124578 (B) 293T whole cell lysates.

VPS28 (S-16) - sc-23210 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

VPS28 (N-12) - sc-23209 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VPS28 (N-12): sc-23209. Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse spleen tissue showing cytoplasmic localization.

VPS28 (FL-221) - sc-30179 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VPS28 FL-221): sc-30179. Western blot analysis of VPS28 expression in non-transfected: sc-117752 (A) and mouse VPS28 transfected: sc-124578 (B) 293T whole cell lysates.

Anti-VPS28 antibody - ab76171 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-VPS28 antibody - ab10133 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-VPS28 antibody - ab37729 from Abcam

Prices $406.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal
Anti-VPS28 antibody (ab37729) at 1/2000 dilution + VPS28 fusion protein at 0.5 µgSecondaryGoat anti-IgY-HRP at 1/1000 dilution

Anti-VPS28 antibody [EPR9880] - ab154793 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9880
Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified) + HepG2 cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-VPS28 antibody [EPR9881(B)] - ab167172 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9881(B)
All lanes : Anti-VPS28 antibody [EPR9881(B)] (ab167172) at 1/1000 dilutionLane 1 : HepG2 cell lysatesLane 2 : Jurkat cell lysatesLane 3 : Human fetal brain lysatesLane 4 : A549 cell lysatesLane 5 : Human fetal liver lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-VPS28 Antibody (Internal) - LS-C95256 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-VPS28 Antibody (aa209-221) - LS-C55673 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-VPS28 Antibody (N-Terminus) - LS-C107818 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
VPS28 antibody LS-C107818 Western blot of Fetal Liver lysate.

Anti-VPS28 Antibody (clone 1A8) - LS-C337944 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A8
IHC of paraffin-embedded Human pancreas tissue using anti-VPS28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

VPS28 Antibody - NBP2-20870 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS28 Antibody [NBP2-20870] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:5000.

VPS28 Antibody - NB100-1399 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: VPS28 Antibody [NB100-1399] - (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

VPS28 Antibody - NBP1-85973 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS28 Antibody [NBP1-85973] - Lane 1: Marker  [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG sp

Anti-VPS28 antibody produced in rabbit - HPA024688 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-VPS28: <B>Cat. No. HPA024688</B>: Immunohistochemical staining of human oral mucosa shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-VPS28 antibody produced in chicken - GW22170F from Sigma-Aldrich

Prices $267.50
Sizes 50 µg
Host Chicken
Clonality Polyclonal

Anti-VPS28, (N-terminal) antibody produced in rabbit - SAB2105015 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-VPS28, antibody produced in rabbit: <B>Cat. No. SAB2105015</B>: Immunoblotting analysis of VPS28 in fetal liver tissue lysate with VPS28 anitbody at 1 μg/mL.

Anti-VPS28 antibody produced in rabbit - HPA024745 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-VPS28: <B>Cat. No. HPA024745</B>: Immunohistochemical staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.

Anti-VPS28 antibody produced in goat - SAB2501104 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-VPS28: <B>Cat. No. SAB2501104</B>: (10 μg/mL) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Anti-VPS28 (171-185) antibody produced in rabbit - V3140 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-VPS28 (171-185): <B>Cat. No. V3140</B>: Immunofluorescence of HUVEC cells using VPS28 (171-185), Cat. No. V3140 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

VPS28 antibody - N-terminal region (ARP56850_P050) - ARP56850_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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