All lanes : Anti-VPS35 antibody [EPR11501(B)] (ab157220) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : Raji cell lysateLane 4 : MOLT4 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VPS35 with ab157220 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling VPS35 with ab157220 at 1/50 dilution.
Flow cytometric analysis of permeabilized MOLT4 cells labeling VPS35 with ab157220 at 1/10 dilution (red) or a rabbit IgG (negative) (green).