VPS35

Gene Symbol VPS35
Entrez Gene 55737
Alt Symbol MEM3, PARK17
Species Human
Gene Type protein-coding
Description vacuolar protein sorting 35 homolog (S. cerevisiae)
Other Description hVPS35|maternal-embryonic 3|vacuolar protein sorting-associated protein 35
Swissprots Q561W2 Q96QK1 Q9H016 Q9NZK2 Q9H8J0 Q9NVG2 Q9NX80 Q9H4P3 Q9NRS7 Q9H096
Accessions EAW82683 EAW82684 Q96QK1 AF086051 AF175265 AAF89953 AF183418 AAG09687 AF186382 AAG40619 AF191298 AAF02778 AK000395 BAA91137 AK001614 BAA91790 AK023650 BAB14626 AK025774 AK223218 BAD96938 AL136888 CAB66822 AL512769 CAC21686 AM393740 CAL38615 AY007112 AAG01989 BC002414 AAH02414 BC010362 AAH10362 BC041367 BC093036 AAH93036 BM971213 BQ574179 BX647213 CAI46268 DB049686 XM_005256045 XP_005256102 XM_011523227 XP_011521529 NM_018206 NP_060676
Function Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved
Subcellular Location Cytoplasm. Membrane; Peripheral membrane protein. Endosome {ECO:0000269|PubMed:15078903}. Early endosome {ECO:0000305}. Late endosome {ECO:0000305}. Note=Localizes to tubular profiles adjacent to endosomes. {ECO:0000269|PubMed:15078903}.
Tissue Specificity Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon.

VPS35 (B-5) - sc-374372 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS35 (B-5): sc-374372. Western blot analysis of VPS35 expression in MOLT-4 (A) and Raji (B) whole cell lysates.

VPS35 (C-20) - sc-55803 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VPS35 (C-20): sc-55803. Western blot analysis of VPS35 expression in Hep G2 (A) and 293T (B) whole cell lysates.

VPS35 (H-300) - sc-134929 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

VPS35 (S-18) - sc-55805 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-VPS35 antibody - ab76329 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-VPS35 antibody - ab57632 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57632 VPS35 antibody at 1ug/ml.

Anti-VPS35 antibody [EPR11501(B)] - ab157220 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11501(B)
All lanes : Anti-VPS35 antibody [EPR11501(B)] (ab157220) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : Raji cell lysateLane 4 : MOLT4 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-VPS35 Antibody (aa190-240) - LS-C95258 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-VPS35 Antibody (C-Terminus) - LS-C345898 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Vps35 Antibody - C-terminal region in Mouse Lung cells lysate.

Anti-VPS35 Antibody (aa783-796) IHC-plus™ - LS-B5909 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5909 concentration 3.75 ug/ml.

Anti-VPS35 Antibody (aa733-796) - LS-C185941 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution:1:250.

VPS35 Antibody - NB100-1397 from Novus Biologicals

Prices $99.00, $329.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western Blot: VPS35 Antibody [NB100-1397] - (1ug/ml) staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

VPS35 Antibody - NBP2-13517 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

VPS35 Antibody - NBP1-31069 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS35 Antibody [NBP1-31069] - A. 30 ug A549 whole cell extract B. 30 ug H1299 whole cell extract C. 30 ug HCT116 whole cell extract 7.5% SDS-PAGE VPS35 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

VPS35 Antibody - NBP2-20876 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.

VPS35 Antibody - NBP1-03427 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

VPS35 Antibody (2D3) - H00055737-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D3
Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in HeLa.

VPS35 Antibody - H00055737-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Monoclonal Anti-VPS35 antibody produced in mouse - WH0055737M2 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D3
<B>Immunoblotting</B><BR/>Monoclonal Anti-VPS35, <B>Cat. No. WH0055737M2</B> used at the antibody concentration: 1 μg/mL. Specific band of ~37.11 kDa using immunogen protein lysate.

Anti-VPS35 (C-terminal) antibody produced in rabbit - SAB2700982 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-VPS35 (C-terminal): <B>Cat. No. SAB2700982</B>: Immunofluorescence analysis of methanol-fixed HCT116, using VPS35 antibody at 1:500 dilution.

Anti-VPS35 (1-15) antibody produced in rabbit - SAB1105015 from Sigma-Aldrich

Prices $279.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HeLa cells was separated on SDS-PAGE, blotted with Anti-VPS35 (1-15) (<B>Cat. No. SAB1105015</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + VPS35 immunizing peptide (human, 1-15)

VPS35 Antibody - C-terminal region (ARP74343_P050) - ARP74343_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

VPS35--MEM3-Antibody - AF2153a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF2153a staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.

VPS35 antibody - orb18631 from Biorbyt

Prices $128.00, $332.00
Sizes 20 μg (Trial Size), 100 μg
Host Goat
Clonality Polyclonal
Immunohistochemical staining of Human Small Intestine using VPS35 antibody

Goat Anti-VPS35 / MEM3 Antibody (OAEB02485) - OAEB02485 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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