Flow cytometric analysis of paraformaldehyde fixed MCF-7 cells labeling VPS4a using ab180581 at 1/30 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution.
Immunocytochemical staining of paraformaldehyde fixed Hela cells labeling VPS4a using ab180581 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution. Cells were counterstained with DAPI (blue)
Immunocytochemical staining of paraformaldehyde fixed MCF-7 cells labeling VPS4a using ab180581 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor®t 488) at 1/200. Cells were counterstainined with DAPI (blue)
Immunohistochemical stainin gof paraffin-embedded Human testis tissue labeling VPS4a with ab180581 at 1/2000 dilution followed by prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG. Sample was counterstained with Hematoxylin.
All lanes : Anti-VPS4a antibody [EPR14545(B)] (ab180581) at 1/50000 dilutionLane 1 : Fetal brain lysateLane 2 : MCF-7 cell lysateLane 3 : Hela cell lysateLane 4 : U87-MG cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated