VPS4A

Gene Symbol VPS4A
Entrez Gene 27183
Alt Symbol SKD1, SKD1A, SKD2, VPS4, VPS4-1
Species Human
Gene Type protein-coding
Description vacuolar protein sorting 4 homolog A (S. cerevisiae)
Other Description SKD1-homolog|hVPS4|vacuolar protein sorting factor 4A|vacuolar protein sorting-associated protein 4A|vacuolar sorting protein 4
Swissprots Q9UN37 B2RCB7 Q8TF07 Q9Y582 Q9UI03
Accessions AAG01470 EAW83261 EAW83262 EAW83263 EAW83264 Q9UN37 AF112215 AAF17203 AF132747 AAL75948 AF155740 AAD42971 AF159063 AAD49227 AF255952 AAK52408 AK000200 BAA91005 AK025850 AK315026 BAG37514 AL133634 CAB63758 BC013021 BC035121 AAH35121 BC047932 AAH47932 EU831747 ACE86581 NM_013245 NP_037377
Function Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphory
Subcellular Location Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Midbody. Note=Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody (PubMed:24814515). Localized in two distinct rings on either side of the Fleming body. {ECO:0000269|PubMed:24814515}.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:10637304, ECO:0000269|PubMed:11563910}.
Top Pathways Endocytosis

VPS4 (C-12) - sc-21463 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VPS4 (C-12): sc-21463. Western blot analysis of VPS4 expression in non-transfected: sc-117752 (A) and human VPS4 transfected: sc-115958 (B) 293T whole cell lysates.

VPS4 (H-165) - sc-32922 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VPS4 (H-165): sc-32922. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic and perinuclear staining.

VPS4 (N-14) - sc-21461 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

VPS4 (E-8) - sc-133122 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS4 (E-8): sc-133122. Western blot analysis of VPS4A expression in non-transfected 293T: sc-117752 (A), human VPS4A transfected 293T: sc-115958 (B), HeLa (C), MCF7 (D), ME-180 (E) and NIH/3T3 (F) whole cell lysates.

Anti-VPS4a antibody - ab177057 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of formalin-fixed paraffin-embedded human brain tissue labeling VPS4a with ab177057 at 1/100 showing cytoplasmic staining

Anti-VPS4a antibody - ab81697 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-VPS4a antibody (ab81697) at 1 µg/ml + fetal muscle lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-VPS4a antibody - ab69415 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-VPS4a antibody (ab69415) at 1/500 dilutionLane 1 : VPS4a transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-VPS4a antibody [EPR14545(B)] - ab180581 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14545(B)
Flow cytometric analysis of paraformaldehyde fixed MCF-7 cells labeling VPS4a using ab180581 at 1/30 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution.

Anti-VPS4a antibody - N-terminal - ab199938 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-VPS4a antibody - N-terminal (ab199938) at 1/1000 dilutionLane 1 : Mouse thymus total cell extractLane 2 : HeLa total cell extract

Anti-VPS4A Antibody (aa38-87) - LS-C81960 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal muscle lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-VPS4A Antibody - LS-C346226 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of A549 cell using VPS4A antibody. Blue: DAPI for nuclear staining.

Anti-VPS4A Antibody (aa74-123) - LS-C81961 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-VPS4A Antibody (aa44-77) - LS-C344619 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysate from HeLa cell line with VPS4A Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

VPS4A Antibody - NBP1-54618 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS4A Antibody [NBP1-54618] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Human Muscle

VPS4A Antibody - NBP1-55140 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS4A Antibody [NBP1-55140] - Titration: 0.2-1 ug/ml, Positive Control: Human brain.

VPS4A Antibody - NBP2-20880 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS4A Antibody [NBP2-20880] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.

VPS4A Antibody - NBP2-20879 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

VPS4A Antibody - H00027183-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: VPS4A Antibody [H00027183-B01P] - Analysis of VPS4A expression in transfected 293T cell line by VPS4A polyclonal antibody. Lane 1: VPS4A transfected lysate(48.07 KDa). Lane 2: Non-transfected lysate.

VPS4a Polyclonal Antibody - bs-7777R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS4 antibody produced in rabbit - SAB4200025 from Sigma-Aldrich

Prices $61.10, $360.00
Sizes 25 µl, 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoprecipitation</B><BR/>Rabbit Anti-VPS4 (<B>Cat. No. SAB4200025</B>) was used to immunoprecipitate VPS4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-VPS4 (<B>Cat. No. SAB4200025</B>) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (<B>Cat. No. R3155</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Positive control: HeLa whole cell extract<BR/>2. 10 μg antibody <BR/>3. 2 μg antibody

Anti-VPS4A (ab2) antibody produced in rabbit - SAB2102684 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS4A antibody produced in mouse - SAB1407341 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-VPS4A: <B>Cat. No. SAB1407341</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~48.1 kDa using VPS4A transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-VPS4 antibody produced in rabbit - SAB2102683 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-VPS4A antibody produced in rabbit - SAB4200022 from Sigma-Aldrich

Prices $61.10, $360.00
Sizes 25 µl, 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoprecipitation</B><BR/>Rabbit Anti-VPS4A (<B>Cat. No. SAB4200022</B>) was used to immunoprecipitate VPS4A from NRK whole cell extract. Detection antibody: Rabbit Anti-VPS4 (<B>Cat. No. SAB4200025</B>) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (<B>Cat. No. R3155</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 10 μg antibody <BR/>2. 5 μg antibody <BR/>3. Negative control: 5 μg nonrelevant antibody

VPS4A antibody - N-terminal region (ARP54915_P050) - ARP54915_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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