Anti-VRK1 antibody [5D1] - N-terminal

• Supplier: Abcam
• Catalog: ab171933
• Prices: $370.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 5D1
• Applications: IHC-P WB IP
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to Human VRK1 aa 1-19 (N terminal) (Cysteine residue)
• Description: Mouse Monoclonal
• Gene: VRK1
• Conjugate: Unconjugated

Product images

Lanes 1 & 4 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/100 dilutionLanes 2 & 5 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLanes 3 & 6 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/1000 dilutionLane 1 : HeLa cell extract (5x10e4 cells)Lane 2 : HeLa cell extract (5x10e4 cells)Lane 3 : HeLa cell extract (5x10e4 cells)Lane 4 : U2OS cell extract (5x10e4 cells)Lane 5 : U2OS cell extract (5x10e4 cells)Lane 6 : U2OS cell extract (5x10e4 cells)SecondaryAlexa488 goat anti-mouse IgGdeveloped using the ECL technique

All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLane 1 : Extracts from TIG1 (5x10e4) cells: Luciferase RNAi (control)Lane 2 : Extracts from TIG1 (5x10e4) cells: VRK1-1 RNAiLane 3 : Extracts from TIG1 (5x10e4) cells: VRK1-2 RNAideveloped using the ECL technique

All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLane 1 : Extracts from HeLa (5x10e4) cells: Luciferase RNAi (control)Lane 2 : Extracts from HeLa (5x10e4) cells: VRK1-1 RNAiLane 3 : Extracts from HeLa (5x10e4) cells: VRK1-2 RNAideveloped using the ECL technique

Immunofluorescent analysis of paraformaldehyde-fixed interphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of paraformaldehyde-fixed metaphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of methanol-fixed interphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of methanol-fixed metaphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

Immunofluorescent analysis of formaldehyde-fixed interphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of formaldehyde-fixed metaphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

Immunofluorescent analysis of methanol-fixed interphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of methanol-fixed metaphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.