VRK1

Gene Symbol VRK1
Entrez Gene 7443
Alt Symbol PCH1, PCH1A
Species Human
Gene Type protein-coding
Description vaccinia related kinase 1
Other Description serine/threonine-protein kinase VRK1|vaccinia virus B1R-related kinase 1|vaccinia-related kinase 1
Swissprots Q99986 Q3SYL2
Accessions EAW81653 EAW81654 Q99986 AB000449 BAA19108 AK290110 BAF82799 BC005970 BC103761 AAI03762 BC112075 AAI12076 BC113510 AAI13511 BU535261 CA436761 XM_006720247 XP_006720310 XM_011537132 XP_011535434 NM_003384 NP_003375
Function Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity. {ECO:0000269|PubMed:10951572, ECO:0000269|PubMed:14645249, ECO:0000269|PubMed:15105425, ECO:0000269|PubMed:16495336, ECO:0000269|PubMed:18617507, ECO:0000269|PubMed:19103756}.
Subcellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle {ECO:0000250}. Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
Tissue Specificity Widely expressed. Highly expressed in fetal liver, testis and thymus. {ECO:0000269|PubMed:9344656}.

VRK1 (1F6) Mouse mAb - 3307 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1F6
Western blot analysis of extracts from 293 and HeLa cells using VRK1 (1F6) Mouse mAb.

VRK1 (A-11) - sc-271061 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VRK1 (A-11): sc-271061. Western blot analysis of VRK1 expression in non-transfected: sc-117752 (A) and human VRK1 transfected: sc-111736 (B) 293T whole cell lysates.

VRK1 (E-3) - sc-390809 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VRK1 (E-3): sc-390809. Western blot analysis of VRK1 expression in non-transfected: sc-117752 (A) and human VRK1 transfected: sc-111736 (B) 293T whole cell lysates.

VRK1 (N-17) - sc-15238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VRK1 (N-17): sc-15238. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts.

VRK1 (Y-16) - sc-15239 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VRK1 (Y-16): sc-15239. Western blot analysis of VRK1 expression in non-transfected: sc-117752 (A) and human VRK1 transfected: sc-111736 (B) 293T whole cell lysates.

VRK1 (1F6) - sc-101554 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VRK1 (1F6): sc-101554. Western blot analysis of VRK1 expression in non-transfected 293T: sc-117752 (A), human VRK1 transfected 293T: sc-111736 (B) and HeLa (C) whole cell lysates.

Anti-VRK1 antibody - ab71686 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VRK1 antibody (ab71686)Lane 1 : Non-transfected 293 cell lysateLane 2 : Transiently transfected 293 cell lysateLysates/proteins at 2 µg per lane.

Anti-VRK1 antibody - ab76864 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-VRK1 antibody (ab76864) at 1/500 dilution + human pancreas tissue lysate at 25 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Anti-VRK1 antibody - ab58050 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab58050 VRK1 antibody at 1ug/ml.

Anti-VRK1 antibody [5D1] - N-terminal - ab171933 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 5D1
Lanes 1 & 4 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/100 dilutionLanes 2 & 5 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLanes 3 & 6 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/1000 dilutionLane 1 : HeLa cell extract (5x10e4 cells)Lane 2 : HeLa cell extract (5x10e4 cells)Lane 3 : HeLa cell extract (5x10e4 cells)Lane 4 : U2OS cell extract (5x10e4 cells)Lane 5 : U2OS cell extract (5x10e4 cells)Lane 6 : U2OS cell extract (5x10e4 cells)SecondaryAlexa488 goat anti-mouse IgGdeveloped using the ECL technique

Anti-VRK1 antibody [5D1] - N-terminal - ab174382 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5D1
Immunocytochemical analysis of methanol fixed Human  interphase cells labelling VRK1 with ab174382 at 1/100 dilution.DNA was stained with DAPI (left) and two images were merged (right; merge).Center and bottom; Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

Anti-VRK1 antibody [EPR10623(B)] - ab151706 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10623(B)
All lanes : Anti-VRK1 antibody [EPR10623(B)] (ab151706) at 1/1000 dilutionLane 1 : MCF7 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.

Anti-VRK1 Antibody (clone 4F9) - LS-C198432 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4F9
Immunofluorescence of monoclonal antibody to VRK1 on HeLa cell . [antibody concentration 10 ug/ml]

Anti-VRK1 Antibody (aa1-396) - LS-C132837 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-VRK1 Antibody (Internal, AP) - LS-C284195 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (Internal, APC) - LS-C284196 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (Internal, Biotin) - LS-C284197 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (Internal, Azide-free, HRP) - LS-C284198 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (Internal, FITC) - LS-C284199 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (Internal, PE) - LS-C284200 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (aa46-292, Biotin) - LS-C300167 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (aa46-292, FITC) - LS-C304467 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (Internal) - LS-C330727 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VRK1 Antibody (aa46-292) - LS-C296933 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant VRK1.

Anti-VRK1 Antibody (aa1-230, clone 2E10) - LS-C339720 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2E10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VRK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VRK1.
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