Anti-VRK1 antibody [5D1] - N-terminal

• Supplier: Abcam
• Catalog: ab174382
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 5D1
• Applications: WB IP IHC ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide within Human VRK1 aa 1-19 (N terminal) (Cysteine residue)
• Description: Mouse Monoclonal
• Gene: VRK1
• Conjugate: Unconjugated

Product images

Immunocytochemical analysis of methanol fixed Human  interphase cells labelling VRK1 with ab174382 at 1/100 dilution.DNA was stained with DAPI (left) and two images were merged (right; merge).Center and bottom; Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

Immunocytochemical analysis of formaldehyde fixed Human U-2OS interphase cells labelling VRK1 with ab174382 at 1/100 dilution.DNA was stained with DAPI (left) and two images were merged (right; merge).Center and bottom; Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

Immunocytochemical analysis of methanol fixed Human HeLa interphase cells labelling VRK1 with ab174382 at 1/100 dilution.DNA was stained with DAPI (left) and two images were merged (right; merge).Center and bottom; Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

Immunocytochemical analysis of paraformaldehyde fixed Human HeLa interphase cells labelling VRK1 with ab174382 at 1/100 dilution. DNA was stained with DAPI (left) and two images were merged (right; merge).Bottom; Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab174382) at 1/500 dilutionLane 1 : Luciferase RNAi (control) Extracts from 50000 cells.Lane 2 : VRK1-1 RNAi Extracts from 50000 cells.Lane 3 : VRK1-2 RNAi Extracts from 50000 cells.developed using the ECL technique

Lanes 1 & 4 : Anti-VRK1 antibody [5D1] - N-terminal (ab174382) at 1/100 dilutionLanes 2 & 5 : Anti-VRK1 antibody [5D1] - N-terminal (ab174382) at 1/500 dilutionLanes 3 & 6 : Anti-VRK1 antibody [5D1] - N-terminal (ab174382) at 1/1000 dilutionLane 1 : HeLa cell extractLane 2 : HeLa cell extractLane 3 : HeLa cell extractLane 4 : U2OS cell extractLane 5 : U2OS cell extractLane 6 : U2OS cell extractLysates/proteins at 50000 cells per lane.SecondaryAlexa488 goat anti-mouse IgGdeveloped using the ECL technique