Anti-WDR34 antibody
| Name | Anti-WDR34 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab81030 |
| Prices | $370.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Horse, Bovine, Cat, Dog, Pig, Xenopus |
| Antigen | Synthetic peptide corresponding to a region within C terminal amino acids 431-480 (QLSLKYLFAV RWSPVRPLVF AAASGKGDVQ LFDLQKSSQK PTVLIKQTQD) of human WDR34 (NP_443076) |
| Description | Rabbit Polyclonal |
| Gene | WDR34 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab81030 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81030, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-WDR34 antibody (ab81030) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Brain lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution