Western blot analysis of extracts from Daudi and Ramos cells, untreated (-) or treated with anti-IgM (12 μg/ml, 10 min; +), using Phospho-DAPP1/BAM32 (Tyr139) (D7G4G) Rabbit mAb (upper), DAPP1/BAM32 (D9K4O) Rabbit mAb #13598 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of phospho-DAPP1/BAM32 (Tyr139) protein from Daudi cells treated with anti-IgM (12 μg/ml, 10 min) using Rabbit DA1E mAb XP ® Isotype Control #3900 (lane 2) or Phospho-DAPP1/BAM32 (Tyr139) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-DAPP1/BAM32 (Tyr139) (D7G4G) Rabbit mAb.