Anti-WDR5 antibody - ChIP Grade
| Name | Anti-WDR5 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab56919 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB ChIP FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 16-121 of Human WDR5 |
| Description | Mouse Monoclonal |
| Gene | WDR5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/20988_WDR5-Primary-antibodies-ab56919-1.jpg)
Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
The interaction of MYC with the trithorax protein ASH2L promotes gene - The interaction of MYC with the trithorax protein ASH2L promotes gene
Ullius A, Luscher-Firzlaff J, Costa IG, Walsemann G, Forst AH, Gusmao EG, Kapelle K, Kleine H, Kremmer E, Vervoorts J, Luscher B. Nucleic Acids Res. 2014 Jun;42(11):6901-20.
Chromatin modifications sequentially enhance ErbB2 expression in ErbB2-positive - Chromatin modifications sequentially enhance ErbB2 expression in ErbB2-positive
Mungamuri SK, Murk W, Grumolato L, Bernstein E, Aaronson SA. Cell Rep. 2013 Oct 31;5(2):302-13.
The chromatin remodeller CHD8 is required for E2F-dependent transcription - The chromatin remodeller CHD8 is required for E2F-dependent transcription
Subtil-Rodriguez A, Vazquez-Chavez E, Ceballos-Chavez M, Rodriguez-Paredes M, Martin-Subero JI, Esteller M, Reyes JC. Nucleic Acids Res. 2014 Feb;42(4):2185-96.
GRHL3/GET1 and trithorax group members collaborate to activate the epidermal - GRHL3/GET1 and trithorax group members collaborate to activate the epidermal
Hopkin AS, Gordon W, Klein RH, Espitia F, Daily K, Zeller M, Baldi P, Andersen B. PLoS Genet. 2012;8(7):e1002829.
LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. - LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing.
Le Douce V, Colin L, Redel L, Cherrier T, Herbein G, Aunis D, Rohr O, Van Lint C, Schwartz C. Nucleic Acids Res. 2012 Mar;40(5):1904-15.
c-Myb binds MLL through menin in human leukemia cells and is an important driver - c-Myb binds MLL through menin in human leukemia cells and is an important driver
Jin S, Zhao H, Yi Y, Nakata Y, Kalota A, Gewirtz AM. J Clin Invest. 2010 Feb;120(2):593-606.
Polycomb group protein displacement and gene activation through MSK-dependent - Polycomb group protein displacement and gene activation through MSK-dependent
Gehani SS, Agrawal-Singh S, Dietrich N, Christophersen NS, Helin K, Hansen K. Mol Cell. 2010 Sep 24;39(6):886-900.