Anti-WDR77 antibody [EPR10708(B)]

• Supplier: Abcam
• Catalog: ab154190
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10708(B)
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human WDR77 (UniProt ID: Q9BQA1)
• Description: Rabbit Monoclonal
• Gene: WDR77
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab154190 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154190, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

ab154190 staining WRD77 (green) in U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS. Samples were incubated with primary antibody (1/150 in PBS + 5% BSA) for 1 hour at 20°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody. Red - DAPI.See Abreview

All lanes : Anti-WDR77 antibody [EPR10708(B)] (ab154190) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : LnCAP cell lysateLane 3 : 293T cell lysateLane 4 : fetal heart lysateLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling WDR77 with ab154190 at 1/50 dilution

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling WDR77 with ab154190 at 1/50 dilution.

Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue using ab154190 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human normal testis tissue using ab154190 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154190 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154190 showing +ve staining.