WDR77

Gene Symbol WDR77
Entrez Gene 79084
Alt Symbol HKMT1069, MEP-50, MEP50, Nbla10071, p44, p44/Mep50
Species Human
Gene Type protein-coding
Description WD repeat domain 77
Other Description WD repeat-containing protein 77|androgen receptor cofactor p44|methylosome protein 50
Swissprots B3KMW6 Q96GK4 Q3LID2 Q9BQA1 B4DP38 Q9BWY3 Q6JZZ5 Q53FU2
Accessions EAW56493 EAW56494 Q9BQA1 AB073603 BAD38641 AB074171 BAE45736 AF052183 AF478464 AAL79917 AK022860 BAG51128 AK223189 BAD96909 AK296163 BAG58902 AK298179 BAG60450 AY225316 AAP79114 BC001679 AAH01679 BC006477 AAH06477 BC009411 AAH09411 BC011778 AAH11778 BC016946 AAH16946 HQ448191 ADQ32672 NM_024102 NP_077007
Function Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. {ECO:0000269|PubMed:11756452, ECO:0000269|PubMed:23071334}.
Subcellular Location Nucleus. Cytoplasm. Note=Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.
Tissue Specificity Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors. {ECO:0000269|PubMed:12972618, ECO:0000269|PubMed:17437848}.

MEP50 Antibody - 2823 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from U2OS and HeLa cells using MEP50 Antibody.

MEP50 (D56B8) Rabbit mAb - 2018 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D56B8
Western blot analysis of extracts of HeLa and U2OS cells using MEP50 (D56B10) Rabbit mAb.

MEP50 (P328) Antibody - 2828 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of U2OS and HeLa cells using MEP50 (Pro328) Antibody.

WDR77 (A-4) - sc-376556 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
WDR77 (A-4): sc-376556. Western blot analysis of WDR77 expression in HeLa (A) and SJRH30 (B) whole cell lysates.

WDR77 (C-2) - sc-376549 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
WDR77 (C-2): sc-376549. Western blot analysis of WDR77 expression in HeLa (A) and SJRH30 (B) whole cell lysates.

WDR77 (FG-4) - sc-100899 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
WDR77 (FG-4): sc-100899. Western blot analysis of WDR77 expression in non-transfected 293T: sc-117752 (A), human WDR77 transfected 293T: sc-112774 (B) and HeLa (C) whole cell lysates.

WDR77 (H-300) - sc-135325 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
WDR77 (H-300): sc-135325. Western blot analysis of WDR77 expression in K-562 (A) and Hep G2 (B) whole cell lysates.

WDR77 (N-14) - sc-163531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
WDR77 (N-14): sc-163531. Western blot analysis of WDR77 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.

WDR77 (Q-13) - sc-163532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

WDR77 (S-16) - sc-163533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-WDR77 antibody - ab85026 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab85026 at 1/100 dilution staining WDR77 in human prostate carcinoma by Immunofluorescence, Formalin-fixed, Paraffin-embedded tissue. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.

Anti-WDR77 antibody - ab57722 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
WDR77 antibody (ab57722) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

Anti-WDR77 antibody - ab66299 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-WDR77 antibody (ab66299) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 8 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-WDR77 antibody [3F10] - ab115596 from Abcam

Prices $464.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3F10
ab115598, at 3µg/ml, staining immunoperoxidase in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry (Immunoperoxidase).

Anti-WDR77 antibody [8A10-C10-E8] - ab184669 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8A10-C10-E8
Immunofluorescence analysis of 1% paraformaldehyde-fixed HeLa cells permeabilized in 0.1% Triton X-100, labeling WDR77 with ab184669 at 1/100 dilution.

Anti-WDR77 antibody [EPR10708(B)] - ab154190 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10708(B)
Overlay histogram showing HeLa cells stained with ab154190 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154190, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-WDR77 antibody [EPR10709(2)] - C-terminal - ab190361 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10709(2)
All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/10000 dilutionLane 1 : LnCaP cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-WDR77 / MEP50 Antibody - LS-C342058 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-WDR77 / MEP50 Antibody (aa1-343, Azide-free) - LS-C329103 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-WDR77 / MEP50 Antibody (N-Terminus) - LS-C112231 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
WDR77 antibody LS-C112231 Western blot of 293T Cell lysate. Antibody concentration 1 ug/ml.

Anti-WDR77 / MEP50 Antibody (aa1-50) - LS-C288357 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human WDR77/MEP50 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-WDR77/MEP50 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.

Anti-WDR77 / MEP50 Antibody - LS-C346245 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of A549 cell using WDR77 antibody. Blue: DAPI for nuclear staining.

Anti-WDR77 / MEP50 Antibody (C-Terminus) - LS-C203124 from LifeSpan Bioscience

Prices $245.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal
Western blot of cell lysates using 1 ug/ml Rabbit Anti-MEP50 Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted Size: 37 KD. Observed Size: 40 KD.

Anti-WDR77 / MEP50 Antibody (aa292-342) - LS-C288358 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human WDR77/MEP50 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-WDR77/MEP50 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Anti-WDR77 / MEP50 Antibody (aa292-342) - LS-C286576 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse WDR77/MEP50 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-WDR77/MEP50 used at a dilution of 1:250.
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