Anti-WDR77 antibody [EPR10709(2)] - C-terminal

• Supplier: Abcam
• Catalog: ab190361
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10709(2)
• Applications: FC IP ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human WDR77 aa 250 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: WDR77
• Conjugate: Unconjugated

Product images

All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/10000 dilutionLane 1 : LnCaP cell lysateLane 2 : 293T cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution + Saos-2 cell lysate at 20 SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH-3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling WDR77 with ab190361 at 1/50 and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown.

Immunofluorescence analysis of A673 cells fixed in 4% paraformaldehyde labeling WDR77 with ab190361 at 1/50 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) 1/200. Counterstained with DAPI. Negative controls also shown.

Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling WDR77 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG used as isotype control.

Immunoprecipitation of HeLa cells labeling WDR77 with ab190361 at 1/50 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000  (+)  or PBS (-)