Anti-Werner's syndrome helicase WRN antibody

• Supplier: Abcam
• Catalog: ab17988
• Prices: $379.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP
• Species Reactivities: Human, Chimpanzee, Monkey, Ape, Orangutan
• Antigen: The epitope recognised to a region between residues 1400 and the C-terminus (residue 1432) of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191
• Description: Rabbit Polyclonal
• Gene: WRN
• Conjugate: Unconjugated

Product images

All lanes : Anti-Werner's syndrome helicase WRN antibody (ab17988) at 1/1000 dilutionLane 1 : Human Lymphocyte whole cell lysateLane 2 : Human Lymphocyte whole cell lysateLysates/proteins at 30 µg per lane.SecondaryAnti Rabbit IgG conjugated to HRP at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Detection of Human WRN by western blot and immunoprecipitation.Lane 1: 100 µg of whole cell lysate from 293T cells.Lane 2: 293T cell lysate immunoprecipated using IgG.Lane 3: 293T cell lysate immunoprecipitated using 5 µg of ab17987 + WRN blocking peptide.Lane 4: 293T cell lysate immunoprecipitated using 5 µg of ab17987.Lane 5: 293T cell lysate immunoprecipitated using 5 µg of ab17988 +WRN blocking peptide.Lane 6:  293T cell lysate immunoprecipitated using 5 µg of ab17988. Lanes 2-6 One 10 cm plate of 293T cells used for immunoprecipitation. Anti-WRN antibody (ab17988) used at 1/10,000 for western blot.Detection:  Chemiluminescence with an exposure time of ~10 seconds.

Product References

Up-regulation of WRN and DNA ligase IIIalpha in chronic myeloid leukemia: - Up-regulation of WRN and DNA ligase IIIalpha in chronic myeloid leukemia:

Sallmyr A, Tomkinson AE, Rassool FV. Blood. 2008 Aug 15;112(4):1413-23.