WRN

Gene Symbol WRN
Entrez Gene 7486
Alt Symbol RECQ3, RECQL2, RECQL3
Species Human
Gene Type protein-coding
Description Werner syndrome, RecQ helicase-like
Other Description DNA helicase, RecQ-like type 3|Werner syndrome ATP-dependent helicase|exonuclease WRN|recQ protein-like 2
Swissprots A1KYY9 Q14191
Accessions AAC41981 AAF06162 AAR05448 EAW63424 EAW63425 EAW63426 Q14191 AB209652 BAD92889 AF091214 AAC63361 AL833572 AY818673 AAX21098 BP279291 XM_011544639 XP_011542941 XM_011544640 XP_011542942 XR_949470 XR_949471 XR_949472 NM_000553 NP_000544
Function Multifunctional enzyme that has both magnesium and ATP- dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double- stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation. {ECO:0000250, ECO:0000269|PubMed:11863428, ECO:0000269|PubMed:17563354, ECO:0000269|PubMed:18596042, ECO:0000269|
Subcellular Location Nucleus, nucleolus. Nucleus. Nucleus, nucleoplasm. Note=Gamma-irradiation leads to its translocation from nucleoli to nucleoplasm and PML regulates the irradiation- induced WRN relocation.

WRN (8H3) Mouse mAb - 4666 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 8H3
Western blot analysis of HeLa, Jurkat, and Ramos cell extracts using WRN (8H3) Mouse mAb.

WRN (D-6) - sc-376182 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
WRN (D-6): sc-376182. Western blot analysis of WRN expression in K-562 nuclear extract.

WRN (C-19) - sc-1956 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
WRN (C-19): sc-1956. Western blot analysis of WRN expression in NAMALWA whole cell lysate.

WRN (96.286) - sc-135808 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

WRN (H-300) - sc-5629 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
WRN (H-300): sc-5629. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.

WRN (1.27) - sc-135807 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
WRN (1.27): sc-135807. Western blot analysis of WRN expression in K-562 nuclear extract.

WRN (F-33) - sc-101110 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
WRN (F-33): sc-101110. Western blot analysis of WRN expression in HeLa nuclear extract.

Anti-Werner's syndrome helicase WRN (phospho S1141) antibody - ab74158 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Werner's syndrome helicase WRN (phospho S1141) antibody (ab74158) at 1/500 dilutionLane 1 : Extracts from K562 cells treated with etoposide (25uM, 24hours)Lane 2 : Extracts from K562 cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-Werner's syndrome helicase WRN antibody - ab200 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab200 (1/1000) staining Werner's syndrome helicase WRN in HeLa cells (green). Cells were fixed in methanol, permeabilized with 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview

Anti-Werner's syndrome helicase WRN antibody - ab17988 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Werner's syndrome helicase WRN antibody (ab17988) at 1/1000 dilutionLane 1 : Human Lymphocyte whole cell lysateLane 2 : Human Lymphocyte whole cell lysateLysates/proteins at 30 µg per lane.SecondaryAnti Rabbit IgG conjugated to HRP at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Werner's syndrome helicase WRN antibody - ab17987 from Abcam

Prices $387.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab17987, at 0.1mcg/ml, staining Human WRN in whole cell lysate (100mcg) in 293T cells by Western blot. WRN was immunoprecipitated with ab17987 at 5mcg/10cm plate (+/- blocking peptide). Detection was by chemiluminescence with an exposure time of less than 10 seconds.

Anti-Werner's syndrome helicase WRN antibody - ab58061 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Werner's syndrome helicase WRN antibody - ab155325 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Werner's syndrome helicase WRN antibody (ab155325) at 1/1000 dilution + A431 whole cell lysate at 30 µg

Anti-Werner's syndrome helicase WRN antibody - ab87270 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab87270, at a 1/250 dilution, staining Werner's syndrome helicase WRN in formalin fixed, paraffin embedded human seminoma tissue by Immunohistochemistry. Detection: DAB staining.

Anti-Werner's syndrome helicase WRN antibody [195C] - ab62747 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 195C

Anti-Werner's syndrome helicase WRN antibody [4H12] - ab66606 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4H12
WRN helicase is illustrated above.The helicase domain is shown in black, and the nuclear localizing signal region is hatched. Epitope sites of 4H12 and 8H3 identified by the studies with various recombinant WRN helicases (Shiratori et. al., J. Cell Biol. 144: 1-9 (1999) are also shown.

Anti-Werner's syndrome helicase WRN antibody [8H3] - ab66601 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8H3
WRN helicase is illustrated above.The helicase domain is shown in black, and the nuclear localizing signal region is hatched. Epitope sites of 4H12 and 8H3 identified by the studies with various recombinant WRN helicases (Shiratori et. al., J. Cell Biol. 144: 1-9 (1999) are also shown.

Anti-Werner's syndrome helicase WRN antibody [EPR6391] - ab133618 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6391
All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6391] (ab133618) at 1/1000 dilutionLane 1 : MOLT4 cell lysates Lane 2 : K562 cell lysates Lane 3 : HeLa cell lysates Lysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Werner's syndrome helicase WRN antibody [EPR6392] - ab124673 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6392
All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilutionLane 1 : MOLT4 cell lysatesLane 2 : K562 cell lysatesLane 3 : A431 cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-Werner's syndrome helicase WRN antibody - C-terminal - ab137325 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Werner's syndrome helicase WRN antibody - C-terminal (ab137325) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-WRN Antibody (C-Terminus) - LS-C90729 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-WRN Antibody (aa400-450) - LS-C90728 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-WRN Antibody - LS-C144273 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-WRN Antibody (aa787-816, AP) - LS-C273637 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WRN Antibody (aa787-816, APC) - LS-C273638 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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