Anti-Werner's syndrome helicase WRN antibody [4H12]

Name	Anti-Werner's syndrome helicase WRN antibody [4H12]
Supplier	Abcam
Catalog	ab66606
Prices	$394.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	4H12
Applications	WB IP ICC/IF
Species Reactivities	Human
Antigen	Human WRN helicase containing C-terminal hexahistidine produced in inscet Sf21 cells by baculovirus expression systems as described by Suzuki et al, and purified by affinity column chromatography using Ni-chelating NTA agarose gel
Description	Mouse Monoclonal
Gene	WRN
Conjugate	Unconjugated
Supplier Page	Shop

Product images

WRN helicase is illustrated above.The helicase domain is shown in black, and the nuclear localizing signal region is hatched. Epitope sites of 4H12 and 8H3 identified by the studies with various recombinant WRN helicases (Shiratori et. al., J. Cell Biol. 144: 1-9 (1999) are also shown.

All lanes : Anti-Werner's syndrome helicase WRN antibody [4H12] (ab66606) at 1/500 dilutionLane 1 : Extracts from cells of normal individualsLane 2 : Extracts from cells of normal individualsLane 3 : Extracts from cells of normal individualsLane 4 : Extracts from cells of patients with Werner syndromeLane 5 : Extracts from cells of patients with Werner syndromeLane 6 : Purified recombinant WRN helicase made by baculovirus technology in the insect SF9 cells. See details published in the paper by Goto et al., Human Genet 105:301-307 (1999).

Immunocytochemical staining of WRN helicase in HeLa cell by ab66606. See details published in the paper by Sakamoto et al., Genes to Cells 6: 421-430 (2001). a. WRN helicase in HeLa cells locating in the nucleoplasm and nucleolus before Camptothecin treatment. b. WRN helicase in HeLa cells forming muclear dots upon double-strand DNA break formation by Camptothecin treatment.

All lanes : Anti-Werner's syndrome helicase WRN antibody [4H12] (ab66606)Lane 1 : Werner syndrome patientLane 2 : Normal individualLane 3 : Normal individual

Product References

BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription. - BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription.

Grierson PM, Lillard K, Behbehani GK, Combs KA, Bhattacharyya S, Acharya S, Groden J. Hum Mol Genet. 2012 Mar 1;21(5):1172-83.