Anti-Werner's syndrome helicase WRN antibody [8H3]

• Supplier: Abcam
• Catalog: ab66601
• Prices: $384.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 8H3
• Applications: ICC/IF ICC/IF IP WB
• Species Reactivities: Human
• Antigen: Human WRN helicase containing N-terminal hexahistidine produced in inscet Sf21 cells by baculovirus expression systems as described by Suzuki et al, and purified by affinity column chromatography using Ni-chelating NTA agarose gel
• Description: Mouse Monoclonal
• Gene: WRN
• Conjugate: Unconjugated

Product images

WRN helicase is illustrated above.The helicase domain is shown in black, and the nuclear localizing signal region is hatched. Epitope sites of 4H12 and 8H3 identified by the studies with various recombinant WRN helicases (Shiratori et. al., J. Cell Biol. 144: 1-9 (1999) are also shown.

All lanes : Anti-Werner's syndrome helicase WRN antibody [8H3] (ab66601) at 1/500 dilutionLane 1 : Extracts from cells of normal individualsLane 2 : Extracts from cells of normal individualsLane 3 : Extracts from cells of normal individualsLane 4 : Extracts of cells from patients with Werner syndromeLane 5 : Extracts of cells from patients with Werner syndromeLane 6 : Purified recombinant WRN helicase made by baculovirus technology in the insect SF9 cells. See details published in the paper by Goto et al., Human Genet 105:301-307 (1999).

ICC/IF image of ab66601 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66601, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Molecular biology of Werner syndrome. - Molecular biology of Werner syndrome.

Shimamoto A, Sugimoto M, Furuichi Y. Int J Clin Oncol. 2004 Aug;9(4):288-98.

Werner helicase relocates into nuclear foci in response to DNA damaging agents - Werner helicase relocates into nuclear foci in response to DNA damaging agents

Sakamoto S, Nishikawa K, Heo SJ, Goto M, Furuichi Y, Shimamoto A. Genes Cells. 2001 May;6(5):421-30.

Detection by epitope-defined monoclonal antibodies of Werner DNA helicases in the - Detection by epitope-defined monoclonal antibodies of Werner DNA helicases in the

Shiratori M, Sakamoto S, Suzuki N, Tokutake Y, Kawabe Y, Enomoto T, Sugimoto M, Goto M, Matsumoto T, Furuichi Y. J Cell Biol. 1999 Jan 11;144(1):1-9.

Immunological diagnosis of Werner syndrome by down-regulated and truncated gene - Immunological diagnosis of Werner syndrome by down-regulated and truncated gene

Goto M, Yamabe Y, Shiratori M, Okada M, Kawabe T, Matsumoto T, Sugimoto M, Furuichi Y. Hum Genet. 1999 Oct;105(4):301-7.

DNA helicase activity in Werner's syndrome gene product synthesized in a - DNA helicase activity in Werner's syndrome gene product synthesized in a

Suzuki N, Shimamoto A, Imamura O, Kuromitsu J, Kitao S, Goto M, Furuichi Y. Nucleic Acids Res. 1997 Aug 1;25(15):2973-8.

Impaired nuclear localization of defective DNA helicases in Werner's syndrome. - Impaired nuclear localization of defective DNA helicases in Werner's syndrome.

Matsumoto T, Shimamoto A, Goto M, Furuichi Y. Nat Genet. 1997 Aug;16(4):335-6.