Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling WHIP with ab99316 at 1/1000 (1µg/ml). Detection: DAB.
Anti-WHIP antibody (ab99316) at 1/1000 dilution + Mouse T cell lysate at 80 µgSecondaryIRDye® 680-conjugated Goat anti-rabbit IgG polyclonal at 1/4000 dilutionPerformed under reducing conditions.
All lanes : Anti-WHIP antibody (ab99316) at 0.4 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
Detection of WHIP by Western Blot of Immunprecipitate. ab99316, at 1 µg/ml, staining WHIP in HeLa whole cell lysate immunoprecipitated using ab99316 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.