All lanes : Anti-WIPF1 antibody (ab98176) at 1 µg/mlLane 1 : Tonsil (Human) Tissue LysateLane 2 : Platelet (Human) Whole Cell LysateLane 3 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 4 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 5 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 6 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.