WIPF1

Gene Symbol WIPF1
Entrez Gene 7456
Alt Symbol PRPL-2, WAS2, WASPIP, WIP
Species Human
Gene Type protein-coding
Description WAS/WASL interacting protein family, member 1
Other Description WAS/WASL-interacting protein family member 1|WASP-interacting protein|Wiskott-Aldrich syndrome protein interacting protein|protein PRPL-2
Swissprots Q6MZU9 B8ZZM1 O43516 Q53TA9 Q9BU37 D3DPE4 Q9UNP1 Q15220
Accessions AAY14708 EAX11129 EAX11130 EAX11131 EAX11132 EAX11133 EAX11134 EAX11135 O43516 AA599214 AA903457 AF031588 AAC03767 AF106062 AAD45972 AK097901 AK301271 BAG62833 AK304194 BAG65074 AL529597 AL832275 BC002914 AAH02914 BC045584 BC110288 AAI10289 BG820214 BM475558 BM542331 BQ016063 BQ441937 BX640870 CAE45928 DA674627 EU832147 ACE87497 EU832240 ACE86810 X86019 CAA60014 XM_011511780 XP_011510082 XM_011511781 XP_011510083 NM_001077269 NP_001070737 NM_003387 NP_003378
Function Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus. {ECO:0000250, ECO:0000269|PubMed:10878810, ECO:0000269|PubMed:19910490, ECO:0000269|PubMed:9405671}.
Subcellular Location Cytoplasmic vesicle {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cell projection, ruffle {ECO:0000269|PubMed:19910490}. Note=Vesicle surfaces and along actin tails. Colocalizes with actin stress fibers. When coexpressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL (By similarity). {ECO:0000250}.
Tissue Specificity Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis. {ECO:0000269|PubMed:9405671}.

WIP (A-7) - sc-271113 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
WIP (A-7): sc-271113. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.

WIP (C-1) - sc-390099 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
WIP (C-1): sc-390099. Western blot analysis of WIP expression in BJAB (A) and HL-60 (B) whole cell lysates.

WIP (E-9) - sc-271114 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
WIP (E-9): sc-271114. Western blot analysis of WIP expression in non-transfected: sc-117752 (A) and human WIP transfected: sc-111797 (B) 293T whole cell lysates.

WIP (82.83) - sc-135806 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

WIP (G-20) - sc-16882 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
WIP (G-20): sc-16882. Immunofluorescence staining of methanol-fixed BJAB cells showing cytoplasmic localization.

WIP (L-15) - sc-23027 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

WIP (H-224) - sc-25533 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
WIP (H-224): sc-25533. Western blot analysis of WIP expression in BJAB whole cell lysate.

WIP (82.6) - sc-135805 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

WIP (S-15) - sc-23026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
WIP (S-15): sc-23026. Western blot analysis of WIP expression in non-transfected: sc-117752 (A) and human WIP transfected: sc-111797 (B) 293T whole cell lysates.

Anti-WIPF1 antibody - ab98176 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-WIPF1 antibody (ab98176) at 1 µg/mlLane 1 : Tonsil (Human) Tissue LysateLane 2 : Platelet (Human) Whole Cell LysateLane 3 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 4 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lane 5 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)Lane 6 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-WIPF1 antibody - ab63944 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-WIPF1 antibody (ab63944) at 0.03 µg/ml + Human tonsil lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-WIPF1 antibody - ab133906 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-WIPF1 antibody (ab133906) at 1 µg/ml + Rat small intestine lysate at 10 µg

Anti-WIPF1 antibody - C-terminal - ab138233 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-WIPF1 antibody - C-terminal (ab138233) at 1/500 dilution + HT-29 cell lysates at 30 µg

Anti-WIPF1 / WIP Antibody (Tyr455) - LS-C193169 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-WIPF1 / WIP Antibody (aa406-455) - LS-C200820 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HT-29 cells using WIPF1 antibody.

Anti-WIPF1 / WIP Antibody (N-Terminus) - LS-C151348 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
WIPF1 / WIP antibody Western Blot of Rat Small Intestine.

Anti-WIPF1 / WIP Antibody (aa478-492) - LS-C55690 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.03 ug/ml) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WIPF1/WIP Antibody - NBP1-86859 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

WIPF1/WIP Antibody - NB100-68147 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: WIPF1/WIP Antibody [NB100-68147] - Analysis of WIPF1/WIP antibody using this antibody at 0.03 ug/ml in Human Tonsil. Primary incubation was 1 hour. Detected by chemiluminescence.

WIPF1/WIP Antibody - 27250002 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-WIPF1 antibody produced in rabbit - HPA003739 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WIPF1: <B>Cat. No. HPA003739</B>: Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp and cells in white pulp.

Anti-WIPF1 antibody produced in goat - SAB2501112 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-WIPF1: <B>Cat. No. SAB2501112</B>: (0.03 μg/mL) staining of Human Tonsil lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WIPF1-Antibody - AF2158a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF2158a (0.03 µg/ml) staining of Human Tonsil lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

WIPF1 Antibody (OAAF07290) - OAAF07290 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

WIPF1 Antibody (OAAF07939) - OAAF07939 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Search more