Anti-WIPI2 antibody [2A2]
| Name | Anti-WIPI2 antibody [2A2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab105459 |
| Prices | $381.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2A2 |
| Applications | ICC/IF ICC/IF IP IHC-P IHC-F WB FC |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide: CSALRLDEDSEHPPMILRTD , corresponding to C terminal amino acids 418-436 of Human WIPI2 (Isoform WIPI2b) |
| Description | Mouse Monoclonal |
| Gene | WIPI2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab105459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105459, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/21375_ab105459-1-ab105459FC.jpg)
Overlay histogram showing HeLa cells stained with ab105459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105459, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen - WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen
Dooley HC, Razi M, Polson HE, Girardin SE, Wilson MI, Tooze SA. Mol Cell. 2014 Jul 17;55(2):238-52.
Pathogenic Parkinson's disease mutations across the functional domains of LRRK2 - Pathogenic Parkinson's disease mutations across the functional domains of LRRK2
Manzoni C, Mamais A, Dihanich S, McGoldrick P, Devine MJ, Zerle J, Kara E, Taanman JW, Healy DG, Marti-Masso JF, Schapira AH, Plun-Favreau H, Tooze S, Hardy J, Bandopadhyay R, Lewis PA. Biochem Biophys Res Commun. 2013 Nov 29;441(4):862-6. doi:
Identification of a candidate therapeutic autophagy-inducing peptide. - Identification of a candidate therapeutic autophagy-inducing peptide.
Shoji-Kawata S, Sumpter R, Leveno M, Campbell GR, Zou Z, Kinch L, Wilkins AD, Sun Q, Pallauf K, MacDuff D, Huerta C, Virgin HW, Helms JB, Eerland R, Tooze SA, Xavier R, Lenschow DJ, Yamamoto A, King D, Lichtarge O, Grishin NV, Spector SA, Kaloyanova DV, Levine B. Nature. 2013 Feb 14;494(7436):201-6.
Autophagy proteins regulate ERK phosphorylation. - Autophagy proteins regulate ERK phosphorylation.
Martinez-Lopez N, Athonvarangkul D, Mishall P, Sahu S, Singh R. Nat Commun. 2013;4:2799.
Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and - Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and
Polson HE, de Lartigue J, Rigden DJ, Reedijk M, Urbe S, Clague MJ, Tooze SA. Autophagy. 2010 May;6(4):506-22.