WIPI2

Gene Symbol WIPI2
Entrez Gene 26100
Alt Symbol ATG18B, Atg21, CGI-50, WIPI-2
Species Human
Gene Type protein-coding
Description WD repeat domain, phosphoinositide interacting 2
Other Description WD repeat domain phosphoinositide-interacting protein 2|WD40 repeat protein interacting with phosphoinositides 2|WIPI49-like protein 2
Swissprots Q9Y4P8 Q9Y364 B3KNC2 Q5MNZ8 Q96IE4 Q75L50 Q6FI96
Accessions AAQ96865 AAQ96866 AAQ96867 EAW87324 EAW87325 EAW87326 EAW87327 EAW87328 Q9Y4P8 AF151808 AAD34045 AI074985 AK023041 BAG51152 AK024279 BAG51284 AK124974 BAC86012 AK125007 AK315468 AL080155 CAB45746 AM392901 CAL37779 AM393618 CAL38494 AM393628 CAL38504 AY691425 AAV80761 AY691426 AAV80762 BC004116 AAH04116 BC007596 AAH07596 BC016912 BC021012 BC021068 BC021200 AAH21200 BI561383 BX537624 CAH56163 CR533530 CAG38561 XM_006715685 XP_006715748 NM_001033518 NP_001028690 NM_001033519 NP_001028691 NM_001033520 NP_001028692 NM_001278299 NP_001265228 NM_015610 NP_056425 NM_016003 NP_057087
Function Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P. {ECO:0000269|PubMed:20505359}.
Subcellular Location Preautophagosomal structure membrane {ECO:0000269|PubMed:20505359, ECO:0000269|PubMed:21564513, ECO:0000269|PubMed:22456507, ECO:0000269|PubMed:23916833}; Peripheral membrane protein {ECO:0000269|PubMed:20505359, ECO:0000269|PubMed:21564513, ECO:0000269|PubMed:22456507}; Cytoplasmic side {ECO:0000269|PubMed:20505359, ECO:0000269|PubMed:21564513, ECO:0000269|PubMed:22456507}. Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P. {ECO:0000269|PubMed:24954904}.
Tissue Specificity Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. {ECO:0000269|PubMed:15602573, ECO:0000269|PubMed:20505359}.

Phospho-WIPI2 (Ser413) Antibody - 13571 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Raji and KARPAS-299 cells, untreated (-) or treated with TPA #4174 (200 nM, 30 min; +) using Phospho-WIPI2 (Ser413) Antibody (upper) or total WIPI2 Antibody #8567 (lower).

WIPI2 Antibody - 8567 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using WIPI2 Antibody.

WIPI-2 (D-20) - sc-83067 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
WIPI-2 (D-20): sc-83067. Western blot analysis of WIPI-2 expression in mouse skeletal muscle tissue extract.

Anti-WIPI2 antibody - ab127736 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-WIPI2 antibody (ab127736) at 1/1000 dilution + A431 whole cell lysate at 30 µg

Anti-WIPI2 antibody - ab139717 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-WIPI2 antibody (ab139717) at 1 µg/ml + Human testis tissue lysate at 15 µg

Anti-WIPI2 antibody [2A2] - ab105459 from Abcam

Prices $381.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2A2
Overlay histogram showing HeLa cells stained with ab105459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105459, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-WIPI2 antibody [EP8112] - ab137024 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP8112
All lanes : Anti-WIPI2 antibody [EP8112] (ab137024) at 1/1000 dilutionLane 1 : Saos-2 lysateLane 2 : BxPC-3 lysateLane 3 : Caco-2 lysateLane 4 : Fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-WIPI2 antibody [EPR8113] - ab131271 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8113
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labelling WIPI2 with ab131271 at 1/100 dilution.

Anti-WIPI2 antibody - C-terminal - ab140278 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-WIPI2 antibody - C-terminal (ab140278) at 1 µg/ml + OVCAR-3 whole cell lysate at 10 µg

Anti-WIPI2 Antibody (C-Terminus, clone 2A2) - LS-C188714 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 2A2

Anti-WIPI2 Antibody (aa426-454, AP) - LS-C273607 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa426-454, APC) - LS-C273608 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa426-454, Biotin) - LS-C273609 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa426-454, FITC) - LS-C273610 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa426-454, Azide-free, HRP) - LS-C273611 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa4-32, AP) - LS-C273613 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa4-32, Biotin) - LS-C273615 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa426-454, PE) - LS-C273612 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa4-32, APC) - LS-C273614 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa4-32, FITC) - LS-C273616 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa4-32, Azide-free, HRP) - LS-C273617 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa4-32, PE) - LS-C273618 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa422-451) - LS-C326365 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa3-32) - LS-C326366 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-WIPI2 Antibody (aa426-454) - LS-C160625 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of WIPI2 Antibody in mouse brain tissue lysates (35 ug/lane). WIPI2 (arrow) was detected using the purified antibody.
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