Anti-WISP1 antibody
| Name | Anti-WISP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10737 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human WISP1 |
| Description | Rabbit Polyclonal |
| Gene | WISP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Image courtesy of Human Protein Atlasab10737 staining WISP1 in human bone marrow, showing staining with haemapoietic tissue and not apidose tissue. Paraffin embedded human bome marrow tissue was incubated with ab10737 (1/75 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab10737 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
All lanes : Anti-WISP1 antibody (ab10737) at 1 µg/mlLane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 4 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab10737 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10737 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Induction of Wnt-inducible signaling protein-1 correlates with invasive breast - Induction of Wnt-inducible signaling protein-1 correlates with invasive breast
Klinke DJ 2nd. PLoS Comput Biol. 2014 Jan;10(1):e1003409.
WISP-1 contributes to fractionated irradiation-induced radioresistance in - WISP-1 contributes to fractionated irradiation-induced radioresistance in
Li WF, Zhang L, Li HY, Zheng SS, Zhao L. PLoS One. 2014 Apr 11;9(4):e94751.
Role of beta-catenin-regulated CCN matricellular proteins in epithelial repair - Role of beta-catenin-regulated CCN matricellular proteins in epithelial repair
Zemans RL, McClendon J, Aschner Y, Briones N, Young SK, Lau LF, Kahn M, Downey GP. Am J Physiol Lung Cell Mol Physiol. 2013 Mar 15;304(6):L415-27. doi: