Anti-XAF1 antibody
| Name | Anti-XAF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab17204 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (Human) (within the last 35 amino acids of C terminal end) |
| Description | Rabbit Polyclonal |
| Gene | XAF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/mlLane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/mlLane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/mlLane 1 : Human spleen lysate Lane 2 : Human spleen lysateLane 3 : Human spleen lysateObserved band size : 32 kDa (why is the actual band size different from the predicted?)Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.
ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC
ICC/IF image of ab17204 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
RNA helicase A is a downstream mediator of KIF1Bbeta tumor-suppressor function in - RNA helicase A is a downstream mediator of KIF1Bbeta tumor-suppressor function in
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Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene - Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene
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XAF1 contributes to dengue virus-induced apoptosis in vascular endothelial cells. - XAF1 contributes to dengue virus-induced apoptosis in vascular endothelial cells.
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Targeted proteasome inhibition by Velcade induces apoptosis in human mesothelioma - Targeted proteasome inhibition by Velcade induces apoptosis in human mesothelioma
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Nuclear translocation of X-linked inhibitor of apoptosis (XIAP) determines cell - Nuclear translocation of X-linked inhibitor of apoptosis (XIAP) determines cell
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