XAF1

Gene Symbol XAF1
Entrez Gene 54739
Alt Symbol BIRC4BP, HSXIAPAF1, XIAPAF1
Species Human
Gene Type protein-coding
Description XIAP associated factor 1
Other Description BIRC4 binding protein|BIRC4-binding protein|XIAP-associated factor 1
Swissprots A2T932 A8K9Y3 Q99982 A8K2L1 A2T931 Q6MZE8 Q8N557 Q6GPH4 D3DTM6
Accessions EAW90287 EAW90288 EAW90289 EAW90290 EAW90291 Q6GPH4 AK056908 BAG51823 AK091799 AK290276 BAF82965 AK292710 BAF85399 AK292848 BAF85537 BC032776 AAH32776 BC058017 BC073156 AAH73156 BM153640 BM459605 BM475564 BQ639060 BX648588 BX649188 CAE46206 EF028165 ABM74556 EF028166 ABM74557 X99699 CAA68030 XM_005256706 XP_005256763 XM_005256708 XP_005256765 XM_011523945 XP_011522247 XM_011523946 XP_011522248 XM_011523947 XP_011522249 XM_011523948 XP_011522250 XM_011523949 XP_011522251 XR_934053 XR_934054 XR_934055 XR_934057 NM_017523 NP_059993 NM_199139 NP_954590 NR_046396 NR_046397 NR_046398
Function Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. {ECO:0000269|PubMed:11175744, ECO:000
Subcellular Location Isoform 5: Nucleus.
Tissue Specificity Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level). {ECO:0000269|PubMed:11175744, ECO:0000269|PubMed:16343440, ECO:0000269|PubMed:17329253, ECO:0000269|PubMed:17570219}.

XAF1 (E1E4O) Rabbit mAb - 13805 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1E4O
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human XAF1 isoform 1 (hXAF1; +), using XAF1 (E1E4O) Rabbit mAb.

XAF1 Antibody - 12615 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

XAF1 (B-5) - sc-398012 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
XAF1 (B-5): sc-398012. Western blot analysis of XAF1 expression in non-transfected: sc-117752 (A) and human XAF1 transfected: sc-111450 (B) 293T whole cell lysates.

XAF1 (A-11) - sc-374020 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
XAF1 (A-11): sc-374020. Western blot analysis of XAF1 expression in non-transfected: sc-117752 (A) and human XAF1 transfected: sc-111450 (B) 293T whole cell lysates.

XAF1 (C-16) - sc-19194 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
XAF1 (C-16): sc-19194. Western blot analysis of XAF1 expression in non-transfected: sc-117752 (A) and human XAF1 transfected: sc-111450 (B) 293T whole cell lysates.

XAF1 (H-4) - sc-374378 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
XAF1 (H-4): sc-374378. Western blot analysis of XAF1 expression in non-transfected: sc-117752 (A) and human XAF1 transfected: sc-111450 (B) 293T whole cell lysates.

XAF1 (H-225) - sc-366039 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

XAF1 (L-16) - sc-19193 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
XAF1 (L-16): sc-19193. Western blot analysis of XAF1 expression in non-transfected: sc-117752 (A) and human XAF1 transfected: sc-111450 (B) 293T whole cell lysates.

Anti-XAF1 antibody - ab17204 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/mlLane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/mlLane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/mlLane 1 : Human spleen lysate Lane 2 : Human spleen lysateLane 3 : Human spleen lysateObserved band size : 32 kDa (why is the actual band size different from the predicted?)Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.

Anti-XAF1 antibody - ab81353 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-XAF1 antibody (ab81353) at 1 µg/mlLane 1 : Tissue lysates prepared from Rat liver tissue.Lane 2 : Tissue lysates prepared from Rat kidney tissue.Lane 3 : Tissue lysates prepared from Rat spleen tissue.Lane 4 : Tissue lysates prepared from Human placenta tissue lysates.Lane 5 : Molecular markerLane 6 : Whole cell lysates prepared from MCF7 cells.Lane 7 : Whole cell lysates prepared from Hela cells.Lane 8 : Whole cell lysates prepared from SMMC cells.Lane 9 : Whole cell lysates prepared from HT1080 cells.Lane 10 : Whole cell lysates prepared from colo320 cells.Lysates/proteins at 50 µg per lane.SecondaryHRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

Anti-XAF1 antibody - ab25889 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-XAF1 antibody (ab25889) at 1 µg/mlLane 2 : Anti-XAF1 antibody (ab25889) at 2 µg/mlLane 3 : Anti-XAF1 antibody (ab25889) at 4 µg/mlLane 1 : Human spleen cell lysate.Lane 2 : Human spleen cell lysate.Lane 3 : Human spleen cell lysate.

Anti-XAF1 antibody - ab100837 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-XAF1 antibody (ab100837) at 1 µg/mlLane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-XAF1 antibody - ab88102 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-XAF1 antibody (ab88102) at 5 µg/ml + Hela nuclear extract at 50 µg

Anti-XAF1 antibody - ab110729 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-XAF1 antibody (ab110729) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-XAF1 antibody - ab98913 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-XAF1 antibody (ab98913) at 1 µg/ml + HeLa cell lysate at 10 µg

Anti-XAF1 antibody - C-terminal - ab199902 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-XAF1 antibody - C-terminal (ab199902) at 1/500 dilutionLane 1 : Mouse brain total cell extractLane 2 : HeLa total cell extract

Anti-XAF1 Antibody (aa235-269) - LS-C344274 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Fluorescent image of HeLa cells stained with XAF1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Anti-XAF1 Antibody IHC-plus™ - LS-B1436 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-XAF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1436 concentration 10 ug/ml.

Anti-XAF1 Antibody (C-Terminus) IHC-plus™ - LS-B618 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-XAF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B618 concentration 2 ug/ml.

Anti-XAF1 Antibody (aa201-218) - LS-C90749 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-XAF1 Antibody (aa306) - LS-C48635 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-XAF1 Antibody (C-Terminus) - LS-C122554 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-XAF1 Antibody (aa283-301) - LS-C122231 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-XAF1 Antibody (C-Terminus, Sepharose) - LS-C172001 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-XAF1 Antibody (aa166-194, APC) - LS-C273661 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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