All lanes : Anti-XAF1 antibody (ab81353) at 1 µg/mlLane 1 : Tissue lysates prepared from Rat liver tissue.Lane 2 : Tissue lysates prepared from Rat kidney tissue.Lane 3 : Tissue lysates prepared from Rat spleen tissue.Lane 4 : Tissue lysates prepared from Human placenta tissue lysates.Lane 5 : Molecular markerLane 6 : Whole cell lysates prepared from MCF7 cells.Lane 7 : Whole cell lysates prepared from Hela cells.Lane 8 : Whole cell lysates prepared from SMMC cells.Lane 9 : Whole cell lysates prepared from HT1080 cells.Lane 10 : Whole cell lysates prepared from colo320 cells.Lysates/proteins at 50 µg per lane.SecondaryHRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
ab81353 at 1µg/ml staining XAF1 in Human endometrium cancer tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded sections). The tissue sections underwent antigen retrieval by heat. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
ICC/IF image of ab81353 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81353, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.