Anti-XBP1 antibody [EPR4086]

• Supplier: Abcam
• Catalog: ab109221
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4086
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human XBP1 aa 100-200
• Description: Rabbit Monoclonal
• Gene: XBP1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HepG2 cells stained with ab109221 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109221, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-XBP1 antibody [EPR4086] (ab109221) at 1/1000 dilutionLane 1 : Fetal heart cell lysates Lane 2 : Fetal kidney cell Fysates Lane 3 : HT-29 cell lysates Lane 4 : 293T cell lysatesLane 5 : Jurkat cell lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human colon tissue using ab109221 at 1/500.

Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue using ab109221 at 1/500.