Anti-XIAP antibody
| Name | Anti-XIAP antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21278 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP |
| Blocking Peptide | Active human XIAP peptide |
| Description | Rabbit Polyclonal |
| Gene | XIAP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-XIAP antibody (ab21278) at 0.5 µg/mlLane 2 : Anti-XIAP antibody (ab21278) at 1 µg/mlLane 3 : Anti-XIAP antibody (ab21278) at 2 µg/mlLane 1 : Human kidney lysate Lane 2 : Human kidney lysate Lane 3 : Human kidney lysate
ab21278 at 2µg/ml staining XIAP in human kidney tissue by IHC
ICC/IF image of ab21278 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21278, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-XIAP antibody (ab21278) at 2 µg/ml + Active human XIAP full length protein (ab95933) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 4 minutes
Product References
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