Anti-XLF antibody
| Name | Anti-XLF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab33499 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Human, Dog |
| Antigen | Synthetic peptide derived from within residues 250 - 350 of Human XLF1 |
| Description | Rabbit Polyclonal |
| Gene | NHEJ1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-XLF antibody (ab33499) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.
![XLF was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to XLF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33499.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: Bands: 35kDa: XLF; non specific - 37kDa: We are unsure as to the identity of this extra band.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/21882_XLF-Primary-antibodies-ab33499-12.jpg)
XLF was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to XLF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33499.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: Bands: 35kDa: XLF; non specific - 37kDa: We are unsure as to the identity of this extra band.
ab33499 (1/200) staining XLF in assynchronous, bleomycin treated, human RPE-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.See Abreview
ICC/IF image of ab33499 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33499, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlasab33499 staining XLF in human kidney. Paraffin embedded human kidney tissue was incubated with ab33499 (1/125 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab33499 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Product References
Canonical non-homologous end joining in mitosis induces genome instability and is - Canonical non-homologous end joining in mitosis induces genome instability and is
Terasawa M, Shinohara A, Shinohara M. PLoS Genet. 2014 Aug 28;10(8):e1004563.
A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA - A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA
Britton S, Coates J, Jackson SP. J Cell Biol. 2013 Aug 5;202(3):579-95.
XRCC4's interaction with XLF is required for coding (but not signal) end joining. - XRCC4's interaction with XLF is required for coding (but not signal) end joining.
Roy S, Andres SN, Vergnes A, Neal JA, Xu Y, Yu Y, Lees-Miller SP, Junop M, Modesti M, Meek K. Nucleic Acids Res. 2012 Feb;40(4):1684-94.
BCR/ABL stimulates WRN to promote survival and genomic instability. - BCR/ABL stimulates WRN to promote survival and genomic instability.
Slupianek A, Poplawski T, Jozwiakowski SK, Cramer K, Pytel D, Stoczynska E, Nowicki MO, Blasiak J, Skorski T. Cancer Res. 2011 Feb 1;71(3):842-51.