NHEJ1

Gene Symbol NHEJ1
Entrez Gene 79840
Alt Symbol XLF
Species Human
Gene Type protein-coding
Description nonhomologous end-joining factor 1
Other Description XRCC4-like factor|non-homologous end-joining factor 1|protein cernunnos
Swissprots Q6IA64 Q96JS9 Q4ZFW7 B8ZZA4 Q9H9Q4
Accessions AAX88921 EAW70678 Q9H9Q4 AJ972687 CAI99410 AK022672 BAB14168 BC008210 AAH08210 BC012732 AAH12732 BC030986 AAH30986 CR457291 CAG33572 DB124934 NM_024782 NP_079058
Function DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. {ECO:0000269|PubMed:16439204, ECO:0000269|PubMed:16439205, ECO:0000269|PubMed:17470781}.
Subcellular Location Nucleus {ECO:0000269|PubMed:16439204, ECO:0000269|PubMed:16439205}.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:16439204}.
Top Pathways Non-homologous end-joining

XLF Antibody - 2854 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines using XLF Antibody.

XLF (E-2) - sc-393844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
XLF (E-2): sc-393844. Western blot analysis of XLF expression in non-transfected: sc-117752 (A) and mouse XLF transfected: sc-124664 (B) 293T whole cell lysates.

XLF (FL-299) - sc-68901 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
XLF (FL-299): sc-68901. Western blot analysis of XLF expression in non-transfected: sc-117752 (A) and mouse XLF transfected: sc-124664 (B) 293T whole cell lysates.

XLF (D-1) - sc-166488 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
XLF (D-1): sc-166488. Western blot analysis of XLF expression in non-transfected: sc-117752 (A) and mouse XLF transfected: sc-124664 (B) 293T whole cell lysates.

XLF (T-17) - sc-79364 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
XLF (T-17): sc-79364. Western blot analysis of XLF expression in non-transfected: sc-117752 (A) and mouse XLF transfected: sc-124664 (B) 293T whole cell lysates.

XLF (D-17) - sc-79361 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

XLF (M-130) - sc-68902 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
XLF (M-130): sc-68902. Western blot analysis of XLF expression in non-transfected: sc-117752 (A) and mouse XLF transfected: sc-124664 (B) 293T whole cell lysates.

Anti-XLF antibody - ab33499 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-XLF antibody (ab33499) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilutionPerformed under reducing conditions.

Anti-XLF antibody - ab129322 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-XLF antibody (ab129322) at 0.1 µg/ml + Human Thyroid lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-XLF antibody - ab62750 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-XLF antibody - ab123443 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-XLF antibody (ab123443) at 1/100 dilution + Hela cell line lysate at 35 µg

Anti-XLF antibody - ab151524 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-XLF antibody (ab151524) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-XLF antibody [1F3] - ab119375 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F3
All lanes : Anti-XLF antibody [1F3] (ab119375) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY XLF cDNALysates/proteins at 5 µg per lane.

Anti-XLF antibody [3E5] - ab117915 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E5
All lanes : Anti-XLF antibody [3E5] (ab117915) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY XLF cDNA Lysates/proteins at 5 µg per lane.

Anti-XLF antibody [EPR15882] - ab189520 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15882
All lanes : Anti-XLF antibody [EPR15882] (ab189520) at 1/20000 dilutionLane 1 : NCCIT cell lysateLane 2 : 293 cell lysateLane 3 : HepG2 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Anti-XLF antibody [EPR15882-36] - C-terminal - ab189917 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15882-36
All lanes : Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917) at 1/10000 dilutionLane 1 : Ramos cell lysateLane 2 : Jurkat cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-XLF antibody [EPR8580(N)] - ab186732 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8580(N)
All lanes : Anti-XLF antibody [EPR8580(N)] (ab186732) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : NCCIT cell lysateLane 3 : HepG2 cell lysateLane 4 : Human placenta lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-XLF antibody - Carboxyterminal end - ab53537 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
ab53537 antibody (0.1µg/ml) staining of XLF in Human Thyroid lysate (35µg protein in RIPA buffer)

Anti-NHEJ1 / XLF Antibody - LS-C48680 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NHEJ1 / XLF Antibody (C-Terminus) - LS-C76889 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NHEJ1 / XLF Antibody (C-Terminus) - LS-C145654 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NHEJ1 antibody LS-C145654 Western blot of ACHN Cell lysate. Antibody concentration 1 ug/ml.

Anti-NHEJ1 / XLF Antibody (clone 3E5) - LS-C115807 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E5
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1.

Anti-NHEJ1 / XLF Antibody (aa268-296) - LS-C164868 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
NHEJ1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The NHEJ1 antibody detected the NHEJ1 protein (arrow).

Anti-NHEJ1 / XLF Antibody (aa267-296, AP) - LS-C264353 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NHEJ1 / XLF Antibody (aa267-296, APC) - LS-C264354 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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