All lanes : Anti-XLF antibody [EPR15882-36] - C-terminal (ab189917) at 1/10000 dilutionLane 1 : Ramos cell lysateLane 2 : Jurkat cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometrical analysis of Ramos cells labeling XLF with ab189917 at 1/60 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of paraformaldehyde-fixed NCCIT cells labeling XLF with ab189917 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).
Immunofluorescent analysis of paraformaldehyde-fixed HepG2 cells labeling XLF with ab189917 at 1/250, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue).
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling XLF with ab189917 at 1/250 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling XLF with ab189917 at 1/250 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.