All lanes : Anti-XLF antibody [EPR8580(N)] (ab186732) at 1/1000 dilutionLane 1 : Ramos cell lysateLane 2 : NCCIT cell lysateLane 3 : HepG2 cell lysateLane 4 : Human placenta lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling XLF with ab186732 at 1/1000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Anti-XLF antibody [EPR8580(N)] (ab186732) at 1/10000 dilution + PC3 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-XLF antibody [EPR8580(N)] (ab186732) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 264.7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution