Anti-XPA antibody
| Name | Anti-XPA antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab85914 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Human, Monkey, Ape, Elephant |
| Antigen | Synthetic peptide, corresponding to a region between residues 1 and 50 of Human XPA (NP_000371 |
| Description | Rabbit Polyclonal |
| Gene | XPA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling XPA with ab85914 at 1/1000 (0.2µg/ml). Detection: DAB.
ICC/IF image of ab85914 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85914, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-XPA antibody (ab85914) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg
Detection of XPA by Western Blot of Immunprecipitate. ab85914 at 0.1µg/ml staining XPA in HeLa whole cell lysate immunoprecipitated using ab85914 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Product References
Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) - Poly(ADP-ribose) contributes to an association between poly(ADP-ribose)
King BS, Cooper KL, Liu KJ, Hudson LG. J Biol Chem. 2012 Nov 16;287(47):39824-33.
Arsenite interacts selectively with zinc finger proteins containing C3H1 or C4 - Arsenite interacts selectively with zinc finger proteins containing C3H1 or C4
Zhou X, Sun X, Cooper KL, Wang F, Liu KJ, Hudson LG. J Biol Chem. 2011 Jul 1;286(26):22855-63.