Anti-XPC antibody [3.26]
| Name | Anti-XPC antibody [3.26] |
|---|---|
| Supplier | Abcam |
| Catalog | ab6264 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3.26 |
| Applications | ICC/IF ICC/IF IHC-P FC WB |
| Species Reactivities | Human |
| Antigen | Recombinant full length human XPC protein expressed in E |
| Description | Mouse Monoclonal |
| Gene | XPC |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Lane 1 : Anti-XPC antibody [3.26] (ab6264) at 1/1000 dilutionLane 2 : Anti-XPC antibody [3.26] (ab6264) at 1/500 dilutionLane 1 : Raji whole cell extractLane 2 : Raji whole cell extract](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/21950_ab6264_3.jpg)
Lane 1 : Anti-XPC antibody [3.26] (ab6264) at 1/1000 dilutionLane 2 : Anti-XPC antibody [3.26] (ab6264) at 1/500 dilutionLane 1 : Raji whole cell extractLane 2 : Raji whole cell extract
Ab6264 staining human XPC in HeLa cells (left), and DAPI staining (right) by immunofluorescence.
ab6264 (4µg/ml) staining XPC in human tonsil, using an automated system (DAKO Autostainer Plus). Using this protocol there is weak nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
![Overlay histogram showing A431 cells stained with ab6264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6264, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/21953_XPC-Primary-antibodies-ab6264-2.jpg)
Overlay histogram showing A431 cells stained with ab6264 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6264, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
FANCJ localization by mismatch repair is vital to maintain genomic integrity - FANCJ localization by mismatch repair is vital to maintain genomic integrity
Guillemette S, Branagan A, Peng M, Dhruva A, Scharer OD, Cantor SB. Cancer Res. 2014 Feb 1;74(3):932-44.
Overexpressed DNA polymerase iota regulated by JNK/c-Jun contributes to - Overexpressed DNA polymerase iota regulated by JNK/c-Jun contributes to
Yuan F, Xu Z, Yang M, Wei Q, Zhang Y, Yu J, Zhi Y, Liu Y, Chen Z, Yang J. PLoS One. 2013 Jul 26;8(7):e69317.
Nucleotide excision repair is not induced in human embryonic lung fibroblasts - Nucleotide excision repair is not induced in human embryonic lung fibroblasts
Rossner P Jr, Mrhalkova A, Uhlirova K, Spatova M, Rossnerova A, Libalova H, Schmuczerova J, Milcova A, Topinka J, Sram RJ. PLoS One. 2013 Jul 19;8(7):e69197.
Targeted gene therapy of xeroderma pigmentosum cells using meganuclease and - Targeted gene therapy of xeroderma pigmentosum cells using meganuclease and
Dupuy A, Valton J, Leduc S, Armier J, Galetto R, Gouble A, Lebuhotel C, Stary A, Paques F, Duchateau P, Sarasin A, Daboussi F. PLoS One. 2013 Nov 13;8(11):e78678.
Repair of UV photolesions in xeroderma pigmentosum group C cells induced by - Repair of UV photolesions in xeroderma pigmentosum group C cells induced by
Kuschal C, DiGiovanna JJ, Khan SG, Gatti RA, Kraemer KH. Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):19483-8. doi:
Recognition of DNA damage by XPC coincides with disruption of the XPC-RAD23 - Recognition of DNA damage by XPC coincides with disruption of the XPC-RAD23
Bergink S, Toussaint W, Luijsterburg MS, Dinant C, Alekseev S, Hoeijmakers JH, Dantuma NP, Houtsmuller AB, Vermeulen W. J Cell Biol. 2012 Mar 19;196(6):681-8.
XPC initiation codon mutation in xeroderma pigmentosum patients with and without - XPC initiation codon mutation in xeroderma pigmentosum patients with and without
Khan SG, Oh KS, Emmert S, Imoto K, Tamura D, Digiovanna JJ, Shahlavi T, Armstrong N, Baker CC, Neuburg M, Zalewski C, Brewer C, Wiggs E, Schiffmann R, Kraemer KH. DNA Repair (Amst). 2009 Jan 1;8(1):114-25.
Spatial separation and bidirectional trafficking of proteins using a - Spatial separation and bidirectional trafficking of proteins using a
Svendsen S, Zimprich C, McDougall MG, Klaubert DH, Los GV. BMC Cell Biol. 2008 Apr 2;9:17.
Xeroderma pigmentosum C is involved in Epstein Barr virus DNA replication. - Xeroderma pigmentosum C is involved in Epstein Barr virus DNA replication.
Lu CC, Chen YC, Wang JT, Yang PW, Chen MR. J Gen Virol. 2007 Dec;88(Pt 12):3234-43.
An aromatic sensor with aversion to damaged strands confers versatility to DNA - An aromatic sensor with aversion to damaged strands confers versatility to DNA
Maillard O, Solyom S, Naegeli H. PLoS Biol. 2007 Apr;5(4):e79.