All lanes : Anti-XRCC3 antibody (ab103070) at 1/250 dilutionLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.