Anti-XRCC3 antibody [XRCC3 10F1/6]
| Name | Anti-XRCC3 antibody [XRCC3 10F1/6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab20254 |
| Prices | $387.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | XRCC3 10F1/6 |
| Applications | WB IHC-P |
| Species Reactivities | Human |
| Antigen | Full length protein (human) which has been his-tagged, overexpressed in E |
| Description | Mouse Monoclonal |
| Gene | XRCC3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab20254 staining human normal placenta. Staining is localized to nuclear compartment.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
