Anti-YAP1 (phospho S127) antibody [EP1675Y]

• Supplier: Abcam
• Catalog: ab76252
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1675Y
• Applications: WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human YAP1 (phospho S127)
• Description: Rabbit Monoclonal
• Gene: YAP1
• Conjugate: Unconjugated

Product images

All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/50000 dilution (purified)Lane 1 : HeLa cell lysate - untreatedLane 2 : HeLa cell lysate - treated with fetal bovine serum and Calyculin ALysates/proteins at 10 µg per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/5000 dilution (unpurified)Lane 1 : C6 treated with Calyculin ALane 2 : NIH/3T3 treated with Calyculin ALysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/50000 dilution (unpurified)Lane 1 : HeLa cell lysates, untreatedLane 2 : HeLa cell lysates, treated with FBS + calyculinLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of endometrium tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling YAP1 (phospho S127) with unpurified ab76252 at 1/100.

Product References

TGF-beta synergizes with defects in the Hippo pathway to stimulate human - TGF-beta synergizes with defects in the Hippo pathway to stimulate human

Fujii M, Toyoda T, Nakanishi H, Yatabe Y, Sato A, Matsudaira Y, Ito H, Murakami H, Kondo Y, Kondo E, Hida T, Tsujimura T, Osada H, Sekido Y. J Exp Med. 2012 Mar 12;209(3):479-94.

Yap controls stem/progenitor cell proliferation in the mouse postnatal epidermis. - Yap controls stem/progenitor cell proliferation in the mouse postnatal epidermis.

Beverdam A, Claxton C, Zhang X, James G, Harvey KF, Key B. J Invest Dermatol. 2013 Jun;133(6):1497-505.

Yes-associated protein 1 is widely expressed in human brain tumors and promotes - Yes-associated protein 1 is widely expressed in human brain tumors and promotes

Orr BA, Bai H, Odia Y, Jain D, Anders RA, Eberhart CG. J Neuropathol Exp Neurol. 2011 Jul;70(7):568-77. doi: