YAP1

Gene Symbol YAP1
Entrez Gene 10413
Alt Symbol COB1, YAP, YAP2, YAP65, YKI
Species Human
Gene Type protein-coding
Description Yes-associated protein 1
Other Description 65 kDa Yes-associated protein|protein yorkie homolog|transcriptional coactivator YAP1|yes-associated protein 2|yes-associated protein YAP65 homolog|yorkie homolog
Swissprots Q7Z574 E3WEB6 E3WEB5 K0L1G3 K0KQ18 Q8IUY9 K0L195 B7ZA01 P46937 E9PRV2 K0KYZ8 F5H202 B4DTY1
Accessions CAA56672 CCH22597 CCH22598 EAW67011 EAW67012 EAW67013 EAW67014 EAW67015 P46937 AA854862 AB567720 BAJ41471 AB567721 BAJ41472 AI128142 AK300414 BAG62143 AK304485 BAG65295 AK310620 AK316116 BAH14487 AL832620 CAD89963 AY316529 AAP92710 BC026212 BC038235 AAH38235 BU073244 CX780993 DC419625 HE864159 CCI79618 HE864160 CCI79619 HE864161 CCI79620 HE864162 CCI79621 HE864163 CCI79622 XM_005271378 XP_005271435 XM_005271380 XP_005271437 XM_005271381 XP_005271438 XM_005271383 XP_005271440 XM_011542555 XP_011540857 XM_011542556 XP_011540858 NM_001130145 NP_001123617 NM_001195044 NP_001181973 NM_001195045 NP_001181974 NM_001282097 NP_001269026 NM_001282098 NP_001269027 NM_001282099 NP_001269028 NM_001282100 NP_001269029 NM_001282101 NP_001269030 NM_006106 NP_006097
Function Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition
Subcellular Location Cytoplasm. Nucleus. Note=Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density. PTPN14 induces translocation from the nucleus to the cytoplasm.
Tissue Specificity Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). {ECO:0000269|PubMed:16461361, ECO:0000269|PubMed:17974916, ECO:0000269|PubMed:7782338}.
Top Pathways Hippo signaling pathway

Phospho-YAP (Ser127) (D9W2I) Rabbit mAb - 13008 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9W2I
Western blot analysis of extracts from PANC-1 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser127) (D9W2I) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb - 13619 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1E7Y
Western blot analysis of extracts from Hep G2 cells, untreated (-) or λ-phosphatase-treated (+), using Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb (upper), YAP Antibody #4912 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). YAP protein isoform 1 Ser397 corresponds to Ser381 of YAP isoform 2, as reported by Zhao et al. (2010) Genes Dev 24, 72-85 (9) .

Phospho-YAP (Ser127) Antibody - 4911 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MDA-MB-468 cells, untreated or treated with wortmannin and LY294002, using Phospho-YAP (Ser127) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.

YAP (D8H1X) XP ® Rabbit mAb - 14074 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8H1X
Western blot analysis of extracts from various cell lines using YAP (D8H1X) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, RL-7 cells are negative for YAP protein expression.

YAP (1A12) Mouse mAb - 12395 from Cell Signaling Technology

Prices $250.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1A12
Western blot analysis of extracts from various cell lines using YAP (1A12) Mouse mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).

YAP Antibody - 4912 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3, C6 and MDA-MB-231 cells, using YAP Antibody.

YAP (B-8) - sc-398182 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
YAP (B-8): sc-398182. Western blot analysis of YAP expression in non-transfected 293T: sc-117752 (A), human YAP transfected 293T: sc-115429 (B) and Caco-2 (C) whole cell lysates.

YAP (H-9) - sc-271134 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
YAP (H-9): sc-271134. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing nuclear and cytoplasmic staining of glandular cells.

YAP (C-20) - sc-17141 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
YAP (C-20): sc-17141. Western blot analysis of YAP expression in non-transfected: sc-117752 (A) and human YAP transfected: sc-115429 (B) 293T whole cell lysate.

YAP (S-20) - sc-17140 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
YAP (S-20): sc-17140. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual cells.

YAP (H-125) - sc-15407 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
YAP (H-125): sc-15407. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovarian cancer tissue showing nuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

YAP (63.7) - sc-101199 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
YAP (63.7): sc-101199. Western blot analysis of YAP expression in non-transfected: sc-117752 (A) and human YAP transfected: sc-115429 (B) 293T whole cell lysate.

Anti-YAP1 (phospho S127) antibody - ab192188 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-YAP1 (phospho S127) antibody (ab192188) at 1/500 dilutionLane 1 : COS7 whole cell lysate with antigen-specific peptideLane 2 : COS7 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-YAP1 (phospho S127) antibody - ab138672 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-YAP1 (phospho S127) antibody (ab138672) at 1/500 dilution + COS7 cell lysate (from cells treated with 2nM HU for 24 hours) at 30 µg

Anti-YAP1 (phospho S127) antibody [EP1675Y] - ab76252 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1675Y
All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252) at 1/50000 dilution (purified)Lane 1 : HeLa cell lysate - untreatedLane 2 : HeLa cell lysate - treated with fetal bovine serum and Calyculin ALysates/proteins at 10 µg per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-YAP1 antibody - ab39361 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of YAP1 staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39361, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-YAP1 antibody - ab56701 from Abcam

Prices $382.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
YAP1 antibody (ab56701) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.

Anti-YAP1 antibody - ab22144 from Abcam

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal

Anti-YAP1 antibody - ab65161 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-YAP1 antibody (ab65161) at 1/500 dilutionLane 1 : Extracts from HepG2 cellsLane 2 : Extracts from COLO205 cellsLane 3 : Extracts from Hela cellsLane 4 : Extracts from Hela cells, plus immunising peptide

Anti-YAP1 antibody - ab56702 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
YAP1 antibody (ab56702) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.

Anti-YAP1 antibody - ab52708 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-YAP1 antibody (ab52708) at 1/1000 dilutionLane 1 : Total protein extract from E coli with ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen.Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein tagged). Lysates/proteins at 20 µg per lane.SecondaryRabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Anti-YAP1 antibody - ab61126 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-YAP1 antibody (ab61126) at 1/500 dilutionLane 1 : HepG2 cell extracts treated with Wortmannin (40nM, 24hours)Lane 2 : HepG2 cell extracts treated with Wortmannin (40nM, 24hours) with immunising peptide

Anti-YAP1 antibody - ab195987 from Abcam

Prices $370.00
Sizes 100 µg
Clonality Polyclonal

Anti-YAP1 antibody [1A12] - ab124474 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A12
All lanes : Anti-YAP1 antibody [1A12] (ab124474) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : C6 cell lysateLane 3 : COS7 cell lysate

Anti-YAP1 antibody [EP1674Y] - ab52771 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1674Y
Anti-YAP1 antibody [EP1674Y] (ab52771) at 1/50000 dilution + Hela cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution
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