Anti-YAP1 antibody [EP1674Y]

• Supplier: Abcam
• Catalog: ab52771
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1674Y
• Applications: WB IP FC IHC-P ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues near the C terminus of human YAP1
• Blocking Peptide: YAP1 peptide
• Description: Rabbit Monoclonal
• Gene: YAP1
• Conjugate: Unconjugated

Product images

Anti-YAP1 antibody [EP1674Y] (ab52771) at 1/50000 dilution + Hela cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using ab52771 at a 1/100 dilution.

developed using the ECL technique

Overlay histogram showing HeLa cells stained with ab52771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52771, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

Crumbs3 is essential for proper epithelial development and viability. - Crumbs3 is essential for proper epithelial development and viability.

Whiteman EL, Fan S, Harder JL, Walton KD, Liu CJ, Soofi A, Fogg VC, Hershenson MB, Dressler GR, Deutsch GH, Gumucio DL, Margolis B. Mol Cell Biol. 2014 Jan;34(1):43-56.

Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent - Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent

Dixit D, Ghildiyal R, Anto NP, Sen E. Cell Death Dis. 2014 May 8;5:e1212.

MEK1 promotes YAP and their interaction is critical for tumorigenesis in liver - MEK1 promotes YAP and their interaction is critical for tumorigenesis in liver

Li L, Wang J, Zhang Y, Zhang Y, Ma L, Weng W, Qiao Y, Xiao W, Wang H, Yu W, Pan Q, He Y, Sun F. FEBS Lett. 2013 Dec 11;587(24):3921-7.

Mutual interaction between YAP and CREB promotes tumorigenesis in liver cancer. - Mutual interaction between YAP and CREB promotes tumorigenesis in liver cancer.

Wang J, Ma L, Weng W, Qiao Y, Zhang Y, He J, Wang H, Xiao W, Li L, Chu Q, Pan Q, Yu Y, Sun F. Hepatology. 2013 Sep;58(3):1011-20.

The tyrosine phosphatase PTPN14 is a negative regulator of YAP activity. - The tyrosine phosphatase PTPN14 is a negative regulator of YAP activity.

Michaloglou C, Lehmann W, Martin T, Delaunay C, Hueber A, Barys L, Niu H, Billy E, Wartmann M, Ito M, Wilson CJ, Digan ME, Bauer A, Voshol H, Christofori G, Sellers WR, Hofmann F, Schmelzle T. PLoS One. 2013 Apr 16;8(4):e61916.

Mutual interaction between YAP and c-Myc is critical for carcinogenesis in liver - Mutual interaction between YAP and c-Myc is critical for carcinogenesis in liver

Xiao W, Wang J, Ou C, Zhang Y, Ma L, Weng W, Pan Q, Sun F. Biochem Biophys Res Commun. 2013 Sep 20;439(2):167-72. doi:

Ajuba family proteins link JNK to Hippo signaling. - Ajuba family proteins link JNK to Hippo signaling.

Sun G, Irvine KD. Sci Signal. 2013 Sep 10;6(292):ra81.

Endocrine-therapy-resistant ESR1 variants revealed by genomic characterization of - Endocrine-therapy-resistant ESR1 variants revealed by genomic characterization of

Li S, Shen D, Shao J, Crowder R, Liu W, Prat A, He X, Liu S, Hoog J, Lu C, Ding L, Griffith OL, Miller C, Larson D, Fulton RS, Harrison M, Mooney T, McMichael JF, Luo J, Tao Y, Goncalves R, Schlosberg C, Hiken JF, Saied L, Sanchez C, Giuntoli T, Bumb C, Cooper C, Kitchens RT, Lin A, Phommaly C, Davies SR, Zhang J, Kavuri MS, McEachern D, Dong YY, Ma C, Pluard T, Naughton M, Bose R, Suresh R, McDowell R, Michel L, Aft R, Gillanders W, DeSchryver K, Wilson RK, Wang S, Mills GB, Gonzalez-Angulo A, Edwards JR, Maher C, Perou CM, Mardis ER, Ellis MJ. Cell Rep. 2013 Sep 26;4(6):1116-30.

Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad - Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad

Wang J, Zhang Y, Weng W, Qiao Y, Ma L, Xiao W, Yu Y, Pan Q, Sun F. J Biol Chem. 2013 Nov 22;288(47):33667-81.

CDK1 phosphorylation of YAP promotes mitotic defects and cell motility and is - CDK1 phosphorylation of YAP promotes mitotic defects and cell motility and is

Yang S, Zhang L, Liu M, Chong R, Ding SJ, Chen Y, Dong J. Cancer Res. 2013 Nov 15;73(22):6722-33.