Anti-YB1 antibody
| Name | Anti-YB1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12148 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB IP |
| Species Reactivities | Mouse, Rat, Human, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human YB1 |
| Blocking Peptide | YB1 peptide |
| Description | Rabbit Polyclonal |
| Gene | YBX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-YB1 antibody (ab12148) at 1 µg/ml + HEK293 Whole Cell Lysate Transiently Overexpressing YB1 at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
All lanes : Anti-YB1 antibody (ab12148) at 1.4 µg/mlLane 1 : HeLa Nuclear lysateLane 2 : HeLa Whole cell lysateLane 3 : MCF-7 cell lysateLane 4 : Jurkat whole cell lysateLane 5 : HEK293 Whole cell lysateLane 6 : HeLa Nuclear lysate with YB1 peptide (ab12411) at 1 µg/mlLane 7 : HeLa Whole cell lysate with YB1 peptide (ab12411) at 1 µg/mlLane 8 : MCF-7 cell lysate with YB1 peptide (ab12411) at 1 µg/mlLane 9 : Jurkat whole cell lysate with YB1 peptide (ab12411) at 1 µg/mlLane 10 : HEK293 whole cell lysate with YB1 peptide (ab12411) at 1 µg/mlLysates/proteins at 20 µg per lane.
ICC/IF image of ab12148 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 5 min) and incubated with the antibody (ab12148, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ICC/IF image of ab12148 stained human HeLa cells. The cells were PFA fixed (3.7% PFA, 5 min) and incubated with the antibody (ab12148, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Image courtesy of Human Protein Atlasab12148 staining YB1 in Human duodenum, showing a distinct and strong staining pattern of the glandular cells. Paraffin embedded human duodenum was incubated with ab12148 (1/5000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.ab12148 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ab12148 was diluted 1/500 and incubated with A2780 whole cell lysate and protein A/G matrix for 16 hours at 4°C to achieve immunoprecipitation. 200 µg of protein was present in the lysate input. Lane 2 shows results of a different antibody used as the negative control. An HRP-conjugated goat anti-rabbit antibody was used for the western blot step.See Abreview
ICC/IF image of ab12148 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12148, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Control of translation and miRNA-dependent repression by a novel poly(A) binding - Control of translation and miRNA-dependent repression by a novel poly(A) binding
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