Anti-YB1 antibody [EP2708Y]
| Name | Anti-YB1 antibody [EP2708Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab76149 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP2708Y |
| Applications | ICC/IF ICC/IF WB IP IHC-P FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues near the C terminus of human YB1 |
| Blocking Peptide | YBX1-Blocking Peptide |
| Description | Rabbit Monoclonal |
| Gene | YBX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-YB1 antibody [EP2708Y] (ab76149) at 1/200000 dilutionLane 1 : HeLa cell lysateLane 2 : SW480 cell lysateLane 3 : A549 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/22238_ab76149wb.gif)
All lanes : Anti-YB1 antibody [EP2708Y] (ab76149) at 1/200000 dilutionLane 1 : HeLa cell lysateLane 2 : SW480 cell lysateLane 3 : A549 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/1000 dilution
Overlay histogram showing HeLa cells stained with ab76149 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76149, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.
![YB1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to YB1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76149.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 46kDa: YB1.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/22240_YB1-Primary-antibodies-ab76149-5.jpg)
YB1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to YB1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76149.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 46kDa: YB1.
ICC/IF image of ab76149 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76149, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab76149 at 1/100 dilution staining YB1 in human kidney by Immunohistochemistry, Paraffin-embedded tissue.
Product References
Mutual regulation between Raf/MEK/ERK signaling and Y-box-binding protein-1 - Mutual regulation between Raf/MEK/ERK signaling and Y-box-binding protein-1
Imada K, Shiota M, Kohashi K, Kuroiwa K, Song Y, Sugimoto M, Naito S, Oda Y. Clin Cancer Res. 2013 Sep 1;19(17):4638-50.
Expression of Y-Box-binding protein 1 in Chinese patients with breast cancer. - Expression of Y-Box-binding protein 1 in Chinese patients with breast cancer.
Xie W, Yang J, Cao Y, Peng C, Ning H, Zhang F, You J. Tumour Biol. 2012 Feb;33(1):63-71.
Foxo3a suppression of urothelial cancer invasiveness through Twist1, - Foxo3a suppression of urothelial cancer invasiveness through Twist1,
Shiota M, Song Y, Yokomizo A, Kiyoshima K, Tada Y, Uchino H, Uchiumi T, Inokuchi J, Oda Y, Kuroiwa K, Tatsugami K, Naito S. Clin Cancer Res. 2010 Dec 1;16(23):5654-63.