Formalin fixed and paraffin embedded human hepatocarcinoma tissue reacted with ab60028 at a dilution of 1/50, which was peroxidase conjugated to the secondary antibody, followed by AEC staining.
Lane 1 : Anti-Yes1 (phospho Y537) antibody (ab60028) at 2.5 µg/mlLane 2 : Anti-Yes1 (phospho Y537) antibody (ab60028) at 1 µg/mlLane 3 : Anti-Yes1 (phospho Y537) antibody (ab60028) at 0.62 µg/mlLane 1 : Cell lysate prepared from human Jurkat cellsLane 2 : Cell l;ysate prepared from human Jurkat cellsLane 3 : Cell lysate prepared from human Jurkat cellsLysates/proteins at 40 µg per lane.developed using the ECL technique