Anti-Zic1 antibody [EPR7291(2)]
| Name | Anti-Zic1 antibody [EPR7291(2)] |
|---|---|
| Supplier | Abcam |
| Catalog | ab134951 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR7291(2) |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues within Human Zic1 (UniProt ID: Q15915) |
| Blocking Peptide | Zic1 peptide |
| Description | Rabbit Monoclonal |
| Gene | ZIC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing SH-SY5Y cells stained with ab134951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134951, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-Zic1 antibody [EPR7291(2)] (ab134951) at 1/1000 dilutionLane 1 : Human cerebellum tissue lysateLane 2 : Human fetal brain tissue lysateLane 3 : SH SY5Y cell lysateLane 4 : SW480 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/23083_Zic1-Primary-antibodies-ab134951-1.JPG)
All lanes : Anti-Zic1 antibody [EPR7291(2)] (ab134951) at 1/1000 dilutionLane 1 : Human cerebellum tissue lysateLane 2 : Human fetal brain tissue lysateLane 3 : SH SY5Y cell lysateLane 4 : SW480 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti rabbit at 1/2000 dilution
Immunofluorescent staining of SW480 cells labelling Zic1 with ab134951 at 1/100 dilution